Buddle B M, McCarthy A R, Ryan T J, Pollock J M, Vordermeier H M, Hewinson R G, Andersen P, de Lisle G W
AgResearch, Wallaceville Animal Research Centre, PO Box 40063, Upper Hutt, New Zealand.
Vet Rec. 2003 Nov 15;153(20):615-20. doi: 10.1136/vr.153.20.615.
More accurate tests are required to test cattle which have reacted positively in the tuberculin skin test. For this purpose, a range of mycobacterial antigens, MPB59, MPB64, MPB70, MPB83, ESAT-6 and CFP10, were used either as recombinant proteins or as synthetic peptides in the whole blood interferon-gamma (IFN-gamma) test. Groups of uninfected cattle with typical 'non-specificity' problems were targeted, in particular animals with skin tuberculosis, animals vaccinated against Johne's disease and animals that were positive in the standard purified protein derivative (PPD)-based IFN-gamma test. The two study groups consisted of 74 Mycobacterium bovis-culture positive animals and 72 uninfected animals, all of which tested positive in the caudal fold tuberculin skin test eight to 28 days before the blood test. The use of combinations of ESAT-6 and CFP10 antigens, either as recombinant proteins or peptides, detected similar percentages of M bovis-infected animals as the PPD-based IFN-gamma test, but produced significantly fewer false positive reactions. The PPD-based IFN-gamma test was very effective in differentiating animals vaccinated against Johne's disease that were skin-test positive from those with bovine tuberculosis, and the use of PPD or specific mycobacterial antigens minimised the number of false positive reactions in animals with skin tuberculosis.
需要更准确的检测方法来检测在结核菌素皮肤试验中呈阳性反应的牛。为此，一系列分枝杆菌抗原，即MPB59、MPB64、MPB70、MPB83、ESAT-6和CFP10，被用作重组蛋白或合成肽用于全血干扰素-γ（IFN-γ）检测。针对具有典型“非特异性”问题的未感染牛群，特别是患有皮肤结核病的动物、接种了副结核菌素疫苗的动物以及在基于标准纯化蛋白衍生物（PPD）的IFN-γ检测中呈阳性的动物。两个研究组包括74头牛分枝杆菌培养阳性动物和72头未感染动物，所有这些动物在血液检测前8至28天的尾褶结核菌素皮肤试验中均呈阳性。使用ESAT-6和CFP10抗原的组合，无论是作为重组蛋白还是肽，检测到的牛分枝杆菌感染动物的百分比与基于PPD的IFN-γ检测相似，但产生的假阳性反应明显较少。基于PPD的IFN-γ检测在区分接种了副结核菌素疫苗且皮肤试验呈阳性的动物和患有牛结核病的动物方面非常有效，并且使用PPD或特定分枝杆菌抗原可将患有皮肤结核病的动物中的假阳性反应数量降至最低。
