Lanzillotta E, Ceccarini C, Ferrara R, Dini F, Frontini F P, Banchetti R
CNR-Istituto di Biofisica, Area della Ricerca, Via Moruzzi 1, Pisa I-56100, Italy.
Sci Total Environ. 2004 Jan 5;318(1-3):211-21. doi: 10.1016/S0048-9697(03)00400-5.
Laboratory experiments on DGM production under light/dark cycles in a culture of the marine diatom Chaetoceros sp. spiked with 200 ng l(-1) of mercury have been performed. DGM formation has been investigated also in the cell exudates, obtained by filtration of the cell culture. Results show that the cell culture and the filtrate give the same value of DGM production (2.24+/-0.88 pg min(-1) l(-1) and 2.23+/-0.02 pg min(-1) l(-1), respectively) in the light (40 W m(-2)), values much higher than to those obtained in the medium culture alone. A significant DGM production has been measured in dark conditions both in the cell culture (0.48+/-0.11 pg min(-1) l(-1)) and in the filtrate (0.85+/-0.10 pg min(-1) l(-1)). The results highlight that the organic compounds released by the cell in the culture medium play a fundamental role in the DGM photo-formation processes.
在添加了200纳克/升汞的海洋硅藻角毛藻属培养物中，进行了在光/暗循环条件下二甲基硫醚（DGM）生成的实验室实验。还对通过细胞培养物过滤获得的细胞渗出物中的DGM形成进行了研究。结果表明，在光照（40瓦/平方米）条件下，细胞培养物和滤液的DGM生成值相同（分别为2.24±0.88皮克/分钟·升和2.23±0.02皮克/分钟·升），这些值远高于仅在培养基培养中获得的值。在黑暗条件下，在细胞培养物（0.48±0.11皮克/分钟·升）和滤液（0.85±0.10皮克/分钟·升）中均检测到显著的DGM生成。结果突出表明，细胞在培养基中释放的有机化合物在DGM光形成过程中起着重要作用。
