Tang Ya-Jie, Zhong Jian-Jiang
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Biotechnol Prog. 2003 Nov-Dec;19(6):1842-6. doi: 10.1021/bp0341592.
Scale-up of a liquid static culture process was studied for hyperproduction of ganoderic acid (GA) by a famous Chinese traditional medicinal mushroom, Ganoderma lucidum. Initial volumetric oxygen transfer coefficient (K(L)a) and area of liquid surface per liquid volume (A(s)) were identified as key factors affecting cell growth and GA accumulation in liquid static cultures of G. lucidum, on the basis of which a multilayer static bioreactor was designed. At a low initial K(L)a level of 2.1 h(-1), a thick layer of white mycelia was formed on the liquid surface, and an optimal production of total GA (i.e., GA production in the liquid and on the liquid surface) was obtained. Both the formation of white mycelia and production of GA on the liquid surface were enhanced with an increase of A(s) within the range as investigated (0.24-1.53 cm(2)/mL). At an A(s) value of 0.90 cm(2)/mL, the total GA production reached maximum. A successful scale-up from a 20-mL static T-flask to a 7.5-L three-layer static bioreactor was achieved based on initial K(L)a. The maximum biomass (20.8 +/- 0.1 g DW/L), GA content (4.96 +/- 0.13 mg/100 mg DW), and total GA production (976 +/- 35 mg/L) were attained in static bioreactors. Not only GA content but also its production obtained in this work were the highest ever reported.
对一种著名的中国传统药用蘑菇灵芝进行液体静态培养过程放大研究，以实现灵芝酸（GA）的高产。初始体积氧传递系数（K(L)a）和单位液体体积的液体表面积（A(s)）被确定为影响灵芝液体静态培养中细胞生长和GA积累的关键因素，并在此基础上设计了一种多层静态生物反应器。在初始K(L)a水平为2.1 h(-1)时，在液体表面形成了一层厚厚的白色菌丝体，并获得了总GA的最佳产量（即液体中和液体表面的GA产量）。在所研究的范围内（0.24 - 1.53 cm(2)/mL），随着A(s)的增加，液体表面白色菌丝体的形成和GA的产量均得到提高。当A(s)值为0.90 cm(2)/mL时，总GA产量达到最大值。基于初始K(L)a，成功地从20 mL静态T形瓶放大到7.5 L三层静态生物反应器。在静态生物反应器中获得了最大生物量（20.8±0.1 g DW/L）、GA含量（4.96±0.13 mg/100 mg DW）和总GA产量（976±35 mg/L）。本研究中获得的GA含量及其产量不仅是有史以来报道的最高值。
