Tang Ya-Jie, Zhang Wei, Zhong Jian-Jiang
Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan, China.
Bioresour Technol. 2009 Mar;100(5):1852-9. doi: 10.1016/j.biortech.2008.10.005. Epub 2008 Nov 17.
Investigations on Ganoderma lucidum fermentation suggested that the responses of the cell growth and metabolites biosynthesis to pH and dissolved oxygen tension (DOT) were different. The ganoderic acid (GA) production of 321.6 mg/L was obtained in the pH-shift culture by combining a 4-day culture at pH 3.0 with the following 6-day culture at pH 4.5, which was higher by 45% and 300% compared with the culture at pH 3.0 and 4.5, respectively. The GA production of 487.1mg/L was achieved in the DOT-shift culture by combining a 6-day culture at 25% of DOT with a following 6-day culture at 10% of DOT, which was higher by 43% and 230% compared with the culture at 25% and 10% of DOT, respectively. A fed-batch fermentation process by combining the above-mentioned pH-shift and DOT-shift strategies resulted in a significant synergistic enhancement of GA accumulation up to 754.6 mg/L, which is the highest reported in the submerged fermentation of G. lucidum in stirred-tank bioreactor.
对灵芝发酵的研究表明，细胞生长和代谢产物生物合成对pH值和溶解氧张力（DOT）的反应有所不同。通过在pH 3.0下培养4天，随后在pH 4.5下培养6天的pH值转换培养，灵芝酸（GA）产量达到321.6 mg/L，分别比在pH 3.0和pH 4.5下培养时高出45%和300%。通过在25%的DOT下培养6天，随后在10%的DOT下培养6天的DOT转换培养，GA产量达到487.1mg/L，分别比在25%和10%的DOT下培养时高出43%和230%。通过结合上述pH值转换和DOT转换策略的分批补料发酵过程，GA积累显著协同增强，高达754.6 mg/L，这是搅拌罐生物反应器中灵芝深层发酵报道的最高产量。
