School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Molecules. 2018 Oct 8;23(10):2563. doi: 10.3390/molecules23102563.
, a famous medicinal mushroom used worldwide, is a rich source of triterpenoids which, together with polysaccharides, are believed to be the main effective constituents of . With the increase of market demand, the wild resource is facing serious limitations, and the quality of cultivated fruiting bodies can be seriously affected by the availability of wood resources and by cultivation management practices. In the present study, we aimed to develop an alternative way to produce useful triterpenoids from . We cultured the strain using a two-stage liquid culture strategy and investigated the effects of nitrogen limitation, carbon supply, static culture volume and air supply in the static culture stage on the accumulation of five triterpenoids (GA-P, GA-Q, GA-T, GA-S, GA-R). Our results showed that, under optimized condition, the total yield of the five triterpenoids reached 963 mg/L (as determined by HPLC). Among the five triterpenoids, GA-T accounted for about 75% of the total yield. Besides, a bioreactor suitable for fungal liquid static culture with a 10 L extensible plastic bag shaped culture unit was designed and in which the maximum total yield of the five GAs reached 856.8 mg/L, and the GAs content reached 5.99%. Our results demonstrate the potential of industrial application of culture for the production of triterpenoids, especially GA-T. Air supply significantly improved the accumulation of triterpenoids, and this will provide important clues to understand why more triterpenoids are produced in the mycelia mat under static liquid culture conditions.
灵芝，一种全球广泛使用的药用蘑菇，是三萜类化合物的丰富来源，与多糖一起，被认为是灵芝的主要有效成分。随着市场需求的增加，野生资源面临严重的限制，而栽培子实体的质量可能会受到木材资源供应和栽培管理实践的严重影响。在本研究中，我们旨在开发一种从灵芝生产有用三萜类化合物的替代方法。我们采用两阶段液体培养策略培养该菌株，并研究了氮限制、碳供应、静置培养体积和静置培养阶段的空气供应对 5 种三萜类化合物（GA-P、GA-Q、GA-T、GA-S、GA-R）积累的影响。结果表明，在优化条件下，5 种三萜类化合物的总产率达到 963mg/L（HPLC 法测定）。在 5 种三萜类化合物中，GA-T 约占总产率的 75%。此外，设计了一种适用于真菌液体静置培养的生物反应器，采用 10L 可扩展塑料袋状培养单元，其中 5 种 GA 的最大总产量达到 856.8mg/L，GA 含量达到 5.99%。我们的结果表明，灵芝液体培养生产三萜类化合物，特别是 GA-T，具有工业应用潜力。空气供应显著提高了三萜类化合物的积累，这将为理解为什么在静置液体培养条件下菌丝垫中会产生更多的三萜类化合物提供重要线索。
