D'Agata Velia, Cavallaro Sebastiano
Institute of Neurological Sciences, Italian National Research Council, CNR, Viale Regina Margherita 6, 95123 Catania, Italy.
Eur J Neurosci. 2003 Nov;18(10):2835-41. doi: 10.1111/j.1460-9568.2003.03025.x.
Many experiments in the past have demonstrated the requirement of de novo gene expression during the long-term retention of learning and memory. Although previous studies implicated individual genes or genetic pathways in learning and memory, the collective behaviours of the genes is mostly unknown. We have used genome-scale screening by microarray analysis to examine the hippocampal expression of more than 1200 genes relevant to neurobiology during instrumental conditioning. Training rats on a step-through passive avoidance task led to unique patterns of gene expression when compared to naïve animals or those exposed to the conditioned or the unconditioned stimulus alone. The newly identified genes afford a quantitative view of the changes which accompany conditioning at the genomic level and enable deeper insights into the molecular basis underlying learning and memory.
过去的许多实验都证明了在学习和记忆的长期保留过程中需要从头开始进行基因表达。尽管先前的研究表明个别基因或基因途径与学习和记忆有关，但这些基因的集体行为大多未知。我们通过微阵列分析进行了全基因组规模的筛选，以检查在操作性条件反射过程中与神经生物学相关的1200多个基因在海马体中的表达。与未受过训练的动物或仅暴露于条件刺激或非条件刺激的动物相比，在穿梭式被动回避任务中训练大鼠会导致独特的基因表达模式。新鉴定出的基因提供了在基因组水平上伴随条件反射的变化的定量视图，并能够更深入地了解学习和记忆的分子基础。
