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海马体中学习诱导的基因表达揭示了神经元-星形胶质细胞代谢偶联在长期记忆中的作用。

Learning-Induced Gene Expression in the Hippocampus Reveals a Role of Neuron -Astrocyte Metabolic Coupling in Long Term Memory.

作者信息

Tadi Monika, Allaman Igor, Lengacher Sylvain, Grenningloh Gabriele, Magistretti Pierre J

机构信息

Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland; Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal, KSA, Saudi Arabia.

出版信息

PLoS One. 2015 Oct 29;10(10):e0141568. doi: 10.1371/journal.pone.0141568. eCollection 2015.

DOI:10.1371/journal.pone.0141568
PMID:26513352
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4625956/
Abstract

We examined the expression of genes related to brain energy metabolism and particularly those encoding glia (astrocyte)-specific functions in the dorsal hippocampus subsequent to learning. Context-dependent avoidance behavior was tested in mice using the step-through Inhibitory Avoidance (IA) paradigm. Animals were sacrificed 3, 9, 24, or 72 hours after training or 3 hours after retention testing. The quantitative determination of mRNA levels revealed learning-induced changes in the expression of genes thought to be involved in astrocyte-neuron metabolic coupling in a time dependent manner. Twenty four hours following IA training, an enhanced gene expression was seen, particularly for genes encoding monocarboxylate transporters 1 and 4 (MCT1, MCT4), alpha2 subunit of the Na/K-ATPase and glucose transporter type 1. To assess the functional role for one of these genes in learning, we studied MCT1 deficient mice and found that they exhibit impaired memory in the inhibitory avoidance task. Together, these observations indicate that neuron-glia metabolic coupling undergoes metabolic adaptations following learning as indicated by the change in expression of key metabolic genes.

摘要

我们研究了与脑能量代谢相关的基因表达，特别是那些在学习后背侧海马体中编码神经胶质细胞（星形胶质细胞）特定功能的基因。使用穿梭式抑制性回避（IA）范式在小鼠中测试情境依赖性回避行为。在训练后3、9、24或72小时或在保持测试后3小时处死动物。mRNA水平的定量测定揭示了学习诱导的、被认为参与星形胶质细胞 - 神经元代谢偶联的基因表达随时间的变化。IA训练后24小时，观察到基因表达增强，特别是编码单羧酸转运蛋白1和4（MCT1、MCT4）、Na/K - ATP酶α2亚基和1型葡萄糖转运蛋白的基因。为了评估这些基因之一在学习中的功能作用，我们研究了MCT1缺陷小鼠，发现它们在抑制性回避任务中表现出记忆受损。总之，这些观察结果表明，如关键代谢基因表达的变化所示，学习后神经元 - 神经胶质细胞代谢偶联经历了代谢适应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/552d851ae184/pone.0141568.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/3d7430f2669e/pone.0141568.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/810b050376c8/pone.0141568.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/90174cb409f5/pone.0141568.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/630046c12108/pone.0141568.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/254001308e7a/pone.0141568.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/2c0f71f70ad8/pone.0141568.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/552d851ae184/pone.0141568.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/3d7430f2669e/pone.0141568.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/810b050376c8/pone.0141568.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/90174cb409f5/pone.0141568.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/630046c12108/pone.0141568.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/254001308e7a/pone.0141568.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/2c0f71f70ad8/pone.0141568.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f11/4625956/552d851ae184/pone.0141568.g007.jpg

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