RNA干扰与mRNA沉默，2004年：它们的应用前景如何？
RNA interference and mRNA silencing, 2004: how far will they reach?
作者信息
Pederson Thoru
机构信息
Department of Biochemistry and Molecular Pharmacology and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
出版信息
Mol Biol Cell. 2004 Feb;15(2):407-10. doi: 10.1091/mbc.e03-10-0726. Epub 2003 Dec 2.
Abstract
The discoveries of RNA interference and RNA-mediated posttranscriptional gene silencing have opened an unanticipated new window on the regulation of gene expression as well as a facile and highly effective tool for knocking down gene expression in many organisms and cells. In addition, RNA interference and RNA silencing may conceivably be exploited for human therapeutics sometime in the future, possibly bringing greater clinical impact than have the so far disappointing antisense endeavors. This essay summarizes recent developments and offers some personalized perspectives, with emphasis on what we do not yet know.
摘要
RNA干扰和RNA介导的转录后基因沉默的发现，为基因表达调控打开了一扇意想不到的新窗口，同时也为在许多生物体和细胞中敲低基因表达提供了一种简便且高效的工具。此外，RNA干扰和RNA沉默在未来某个时候有望被用于人类治疗，可能会带来比迄今为止令人失望的反义技术更大的临床影响。本文总结了近期的进展并提供了一些个人观点，重点在于我们尚不了解的方面。
相似文献
1
RNA interference and mRNA silencing, 2004: how far will they reach?RNA干扰与mRNA沉默，2004年：它们的应用前景如何？
Mol Biol Cell. 2004 Feb;15(2):407-10. doi: 10.1091/mbc.e03-10-0726. Epub 2003 Dec 2.
2
RNA interference: historical overview and significance.RNA干扰：历史概述与意义
Methods Mol Biol. 2004;265:3-21. doi: 10.1385/1-59259-775-0:003.
3
D-RNAi (messenger RNA-antisense DNA interference) as a novel defense system against cancer and viral infections.D-RNAi（信使核糖核酸-反义脱氧核糖核酸干扰）作为一种对抗癌症和病毒感染的新型防御系统。
Curr Cancer Drug Targets. 2001 Nov;1(3):241-7. doi: 10.2174/1568009013334151.
4
Posttranscriptional gene silencing.
Cell. 2007 Aug 10;130(3):570. doi: 10.1016/j.cell.2007.07.042.
5
Pseudouridine Chemical Labeling and Profiling.假尿苷化学标记与分析
Methods Enzymol. 2015;560:247-72. doi: 10.1016/bs.mie.2015.03.010. Epub 2015 Jun 2.
6
The Arabidopsis DCP2 gene is required for proper mRNA turnover and prevents transgene silencing in Arabidopsis.拟南芥 DCP2 基因对于 mRNA 的正确代谢至关重要，并能防止拟南芥中转基因沉默。
Plant J. 2012 Nov;72(3):368-77. doi: 10.1111/j.1365-313X.2012.05066.x. Epub 2012 Aug 30.
7
Bottlenecks in development of retinal therapeutic post-transcriptional gene silencing agents.视网膜治疗性转录后基因沉默剂开发中的瓶颈
Vision Res. 2008 Feb;48(3):453-69. doi: 10.1016/j.visres.2007.09.011. Epub 2007 Oct 31.
8
Posttranscriptional Regulation of the Inflammatory Marker C-Reactive Protein by the RNA-Binding Protein HuR and MicroRNA 637.RNA结合蛋白HuR和微小RNA 637对炎症标志物C反应蛋白的转录后调控
Mol Cell Biol. 2015 Dec;35(24):4212-21. doi: 10.1128/MCB.00645-15. Epub 2015 Oct 5.
9
Advances in lipid-based platforms for RNAi therapeutics.基于脂质的 RNAi 治疗药物载体的研究进展。
J Med Chem. 2014 Feb 27;57(4):1138-46. doi: 10.1021/jm400791q. Epub 2013 Oct 21.
10
About the nature of RNA interference.关于RNA干扰的性质。
Appl Microbiol Biotechnol. 2005 Jun;67(4):429-35. doi: 10.1007/s00253-004-1882-1. Epub 2005 Feb 10.
引用本文的文献
1
Introns and Their Therapeutic Applications in Biomedical Researches.内含子及其在生物医学研究中的治疗应用。
Iran J Biotechnol. 2023 Oct 1;21(4):e3316. doi: 10.30498/ijb.2023.334488.3316. eCollection 2023 Oct.
2
Therapeutic potential of a combination of two gene-specific small interfering RNAs against clinical strains of Acanthamoeba.针对棘阿米巴临床株的两种基因特异性小干扰 RNA 联合的治疗潜力。
Antimicrob Agents Chemother. 2010 Dec;54(12):5151-5. doi: 10.1128/AAC.00329-10. Epub 2010 Sep 20.
3
New strategies in drug discovery.药物研发中的新策略。
Methods Mol Biol. 2006;316:1-11. doi: 10.1385/1-59259-964-8:1.
本文引用的文献
1
Human let-7 stem-loop precursors harbor features of RNase III cleavage products.人类let-7茎环前体具有核糖核酸酶III切割产物的特征。
Nucleic Acids Res. 2003 Nov 15;31(22):6593-7. doi: 10.1093/nar/gkg855.
2
Functional genomics of cell morphology using RNA interference: pick your style, broad or deep.利用RNA干扰技术对细胞形态进行功能基因组学研究：选择你的方式，广度或深度。
J Biol. 2003;2(4):25. doi: 10.1186/1475-4924-2-25. Epub 2003 Oct 1.
3
The nuclear RNase III Drosha initiates microRNA processing.细胞核核糖核酸酶III Drosha启动微小RNA的加工过程。
Nature. 2003 Sep 25;425(6956):415-9. doi: 10.1038/nature01957.
4
A microRNA array reveals extensive regulation of microRNAs during brain development.一个微小RNA阵列揭示了大脑发育过程中微小RNA的广泛调控。
RNA. 2003 Oct;9(10):1274-81. doi: 10.1261/rna.5980303.
5
An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei.在布氏锥虫中发现一种与多核糖体相关的小干扰RNA核糖核蛋白。
RNA. 2003 Jul;9(7):802-8. doi: 10.1261/rna.5270203.
6
Small nucleolar RNA interference induced by antisense or double-stranded RNA in trypanosomatids.反义或双链RNA在锥虫中诱导的小核仁RNA干扰
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7521-6. doi: 10.1073/pnas.1332001100. Epub 2003 Jun 13.
7
RNA polymerase III transcripts and the PTB protein are essential for the integrity of the perinucleolar compartment.RNA聚合酶III转录本和PTB蛋白对于核仁周围区域的完整性至关重要。
Mol Biol Cell. 2003 Jun;14(6):2425-35. doi: 10.1091/mbc.e02-12-0818.
8
Imprinted microRNA genes transcribed antisense to a reciprocally imprinted retrotransposon-like gene.印记微小RNA基因以反义方向转录，与一个相互印记的反转录转座子样基因相对。
Nat Genet. 2003 Jul;34(3):261-2. doi: 10.1038/ng1171.
9
Programmed DNA deletion as an RNA-guided system of genome defense.作为一种RNA引导的基因组防御系统的程序性DNA缺失
Science. 2003 Jun 6;300(5625):1581-4. doi: 10.1126/science.1084737.
10
Molecular biology. A self-help guide for a trim genome.分子生物学。精简基因组自助指南。
Science. 2003 Jun 6;300(5625):1517-8. doi: 10.1126/science.1086053.
Zotero 插件