人巨细胞病毒实验室菌株和临床菌株的编码潜能
Coding potential of laboratory and clinical strains of human cytomegalovirus.
作者信息
Murphy Eain, Yu Dong, Grimwood Jane, Schmutz Jeremy, Dickson Mark, Jarvis Michael A, Hahn Gabriele, Nelson Jay A, Myers Richard M, Shenk Thomas E
机构信息
Department of Molecular Biology, Princeton University, Princeton, NJ 80544, USA.
出版信息
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14976-81. doi: 10.1073/pnas.2136652100. Epub 2003 Dec 1.
Abstract
Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.
摘要
已对六株人巨细胞病毒进行了测序，其中包括两株在成纤维细胞中广泛传代的实验室菌株（AD169和Towne）以及四株在实验室中有限传代的临床分离株（托莱多、FIX、PH和TR）。所有已测序的病毒基因组均已克隆为具有感染性的细菌人工染色体。已鉴定出总共252个具有编码蛋白质潜力的开放阅读框，这些开放阅读框在该病毒的所有四株临床分离株中都是保守的。多序列比对显示，许多保守开放阅读框所编码的氨基酸序列存在显著差异。
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本文引用的文献
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