Davison Andrew J, Dolan Aidan, Akter Parvis, Addison Clare, Dargan Derrick J, Alcendor Donald J, McGeoch Duncan J, Hayward Gary S
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
Molecular Virology Laboratories, Oncology Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA.
J Gen Virol. 2003 Jan;84(Pt 1):17-28. doi: 10.1099/vir.0.18606-0.
The gene complement of wild-type human cytomegalovirus (HCMV) is incompletely understood, on account of the size and complexity of the viral genome and because laboratory strains have undergone deletions and rearrangements during adaptation to growth in culture. We have determined the sequence (241 087 bp) of chimpanzee cytomegalovirus (CCMV) and have compared it with published HCMV sequences from the laboratory strains AD169 and Toledo, with the aim of clarifying the gene content of wild-type HCMV. The HCMV and CCMV genomes are moderately diverged and essentially collinear. On the basis of conservation of potential protein-coding regions and other sequence features, we have discounted 51 previously proposed HCMV ORFs, modified the interpretations for 24 (including assignments of multiple exons) and proposed ten novel genes. Several errors were detected in the published HCMV sequences. We presently recognize 165 genes in CCMV and 145 in AD169; this compares with an estimate of 189 unique genes for AD169 made in 1990. Our best estimate for the complement of wild-type HCMV is 164 to 167 genes.
由于病毒基因组的大小和复杂性，以及实验室毒株在适应细胞培养生长过程中发生了缺失和重排，野生型人类巨细胞病毒（HCMV）的基因组成尚未完全明确。我们测定了黑猩猩巨细胞病毒（CCMV）的序列（241087 bp），并将其与实验室毒株AD169和托莱多株已发表的HCMV序列进行比较，目的是阐明野生型HCMV的基因组成。HCMV和CCMV基因组中度分化且基本共线。基于潜在蛋白质编码区和其他序列特征的保守性，我们否定了先前提出的51个HCMV开放阅读框（ORF），修正了24个（包括多个外显子的归属）的解读，并提出了10个新基因。在已发表的HCMV序列中检测到了几处错误。我们目前认定CCMV中有165个基因，AD169中有145个基因；相比之下，1990年对AD169独特基因的估计为189个。我们对野生型HCMV基因组成的最佳估计是164至167个基因。
