Sucić M, Boban D, Marković-Glamocak M, Jakić-Razumović J, Vrbanec D, Ries S, Gjadrov-Kuvezdić K, Cobanov A M, Podolski P, Martinović I, Erak V, Budisić Z, Ivrlac R, Unusić J
Department of Cytology, Clinical Institute of Laboratory Diagnosis, Zagreb University Hospital Center and School of Medicine, Kispaticeva, Zagreb, Croatia.
Breast. 2003 Feb;12(1):51-7. doi: 10.1016/s0960-9776(02)00281-3.
Numerous studies have demonstrated that the levels of cyclooxygenase (COX), the enzyme that catalyzes the conversion of arachidonic acid to prostaglandin H(2), and of prostaglandins are higher in various tumors and cells during inflammation than in normal tissues. The aim of the present study was to analyze whether COX-2 isoform expression was noticeably higher in fine-needle aspirates (FNA) from breast carcinoma than in FNA from fibroadenoma and fibrocystic breast tissue. COX-2 expression was detected by immunocytochemical (IC) staining and was analyzed by microscopic scoring and computer gray-scale analysis. Evaluation of COX-2 IC positivity in FNA from three groups of patients (nine with breast carcinoma, nine with fibroadenoma, eight with fibrocystic breasts) revealed high COX-2 IC positivity in the majority of patients with breast carcinoma and low or absent COX-2 IC positivity in patients with fibrocystic breast changes. In addition, low or medium COX-2 IC positivity was found in the majority of patients with fibroadenoma, only three of these patients having high COX-2 IC positivity.
众多研究表明，在炎症期间，催化花生四烯酸转化为前列腺素H2的环氧化酶（COX）以及前列腺素在各种肿瘤和细胞中的水平高于正常组织。本研究的目的是分析乳腺癌细针穿刺抽吸物（FNA）中环氧化酶-2（COX-2）同工型的表达是否明显高于纤维腺瘤和乳腺纤维囊性组织的FNA。通过免疫细胞化学（IC）染色检测COX-2的表达，并通过显微镜评分和计算机灰度分析进行分析。对三组患者（9例乳腺癌患者、9例纤维腺瘤患者、8例乳腺纤维囊性变患者）的FNA进行COX-2 IC阳性评估，结果显示大多数乳腺癌患者COX-2 IC呈高阳性，而乳腺纤维囊性变患者COX-2 IC呈低阳性或阴性。此外，大多数纤维腺瘤患者COX-2 IC呈低阳性或中等阳性，其中只有3例患者COX-2 IC呈高阳性。
