Banerjee S, Figueiredo F C, Easty D L, Dick A D, Nicholls S M
Division of Ophthalmology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK.
Br J Ophthalmol. 2003 Dec;87(12):1515-22. doi: 10.1136/bjo.87.12.1515.
To investigate the development of lymphoid aggregates in the conjunctiva after corneal transplantation in rats.
LEW or PVG strain corneas were transplanted orthotopically to PVG rats. Cornea and conjunctiva were examined clinically for up to 42 days. Eyes were removed with attached conjunctiva on days 10 and 15 after transplantation (before and during rejection), together with normal eyes, fixed, paraffin embedded, and examined immunohistochemically.
Clinically, the temporal half of the upper palpebral conjunctiva of recipients of 10/19 allografts and 1/10 isografts developed pronounced swelling, correlating with inflammation and rejection. Histologically, the swelling comprised leucocytic aggregates with an altered overlying epithelium. Aggregates contained granulocytes, macrophages, and cells expressing major histocompatibility complex (MHC) class II, CD4, and CD8, all more numerous in allograft associated conjunctiva. Class II+ cells were more abundant at the surface, whereas macrophages and T cells were more numerous in the deeper stroma. There were few B cells. There was greater CD54 expression by vascular endothelium in allograft associated aggregates. Cells expressing TNFalpha and IFNgamma but not IL1beta were present in stromal and superficial areas.
Corneal transplantation in rats induces the development of organised conjunctival leucocytic aggregates in a fixed location that are significantly more pronounced in recipients of allografts compared with isografts and show characteristics of a Th1 type immune response. These aggregates have characteristics of conjunctiva associated lymphoid tissue and may be sites of presentation of graft antigens and lymphocyte proliferation at the ocular surface.
研究大鼠角膜移植后结膜中淋巴样聚集物的发育情况。
将LEW或PVG品系的角膜原位移植到PVG大鼠。对角膜和结膜进行长达42天的临床检查。在移植后第10天和第15天（排斥反应前和排斥反应期间），连同正常眼一起摘除带有附着结膜的眼球，固定、石蜡包埋，并进行免疫组织化学检查。
临床上，10/19同种异体移植和1/10同基因移植受体的上睑结膜颞侧半出现明显肿胀，与炎症和排斥反应相关。组织学上，肿胀由白细胞聚集物和上方改变的上皮组成。聚集物包含粒细胞、巨噬细胞以及表达主要组织相容性复合体（MHC）II类、CD4和CD8的细胞，在同种异体移植相关结膜中这些细胞数量更多。II类+细胞在表面更丰富，而巨噬细胞和T细胞在更深的基质中数量更多。B细胞很少。同种异体移植相关聚集物中血管内皮的CD54表达更高。在基质和浅表区域存在表达肿瘤坏死因子α和干扰素γ但不表达白细胞介素1β的细胞。
大鼠角膜移植诱导在固定位置形成有组织的结膜白细胞聚集物，与同基因移植相比，同种异体移植受体中的这些聚集物明显更显著，并表现出Th1型免疫反应的特征。这些聚集物具有结膜相关淋巴样组织的特征，可能是眼表移植抗原呈递和淋巴细胞增殖的部位。
