Salles M W, Middleton D M
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Sask.S7N 5B4 Canada.
Vet Immunol Immunopathol. 2000 Nov 23;77(1-2):133-44. doi: 10.1016/s0165-2427(00)00231-2.
The palatine tonsils are part of the mucosa-associated lymphoid tissue (MALT), strategically located in the oropharynx at the entrance of respiratory and gastrointestinal tracts, and are recognized portals of entry and sites of multiplication and persistence of several pathogens in pigs. As the tonsillar crypt epithelium is in close contact with external environment and the underlying lymphoid tissue, the characterization of the intra-epithelial lymphocyte subpopulations is essential for the understanding of initial steps of pathogenesis of several diseases. In this work we investigated specific lymphocyte subsets in the tonsillar crypt epithelium of 10 adult healthy pigs, using monoclonal antibodies against lymphocyte markers CD3, CD4, CD8, gammadelta T cell receptor and immunoglobulin light-chain in an avidin-biotin immunoperoxidase technique. The crypt epithelium was usually extensively infiltrated by a diverse population of T cells and by B cells. The degree of infiltration of each subset was variable among animals and within individual animals. In the T cell population CD4 cells and gammadelta TCR cells predominated over CD8 cells. These data suggest that the crypt lymphoepithelium is capable of participating in both cellular and humoral immune responses and that gammadelta T cells may play an important role in the defense of this mucosa.
腭扁桃体是黏膜相关淋巴组织（MALT）的一部分，位于口咽部呼吸道和胃肠道入口的战略位置，是猪体内几种病原体公认的进入门户、增殖和持续存在的部位。由于扁桃体隐窝上皮与外部环境及下方的淋巴组织密切接触，因此上皮内淋巴细胞亚群的特征对于理解几种疾病发病机制的初始步骤至关重要。在本研究中，我们采用抗淋巴细胞标志物CD3、CD4、CD8、γδ T细胞受体和免疫球蛋白轻链的单克隆抗体，通过抗生物素蛋白-生物素免疫过氧化物酶技术，对10头成年健康猪的扁桃体隐窝上皮中的特定淋巴细胞亚群进行了研究。隐窝上皮通常被不同群体的T细胞和B细胞广泛浸润。每个亚群的浸润程度在不同动物之间以及单个动物体内都有所不同。在T细胞群体中，CD4细胞和γδ TCR细胞比CD8细胞占优势。这些数据表明，隐窝淋巴上皮能够参与细胞免疫和体液免疫反应，并且γδ T细胞可能在这种黏膜的防御中发挥重要作用。
