[导致贝加尔湖（西伯利亚海豹）和里海（里海海豹）海豹 epizooty 的麻疹病毒的分子遗传学比较]

[Molecular-genetic comparison of morbilliviruses which caused epizooty in Baikal (Phoca siberica) and Caspian (Phoca caspica) seals].

作者信息

Butina T V, Denikina N N, Kondratov I G, Belikov S I, Durymanova A A, Blinov V M, Shestopalov A M

出版信息

Mol Gen Mikrobiol Virusol. 2003(4):27-32.

Abstract

The nucleotide sequences were determined for a phosphoprotein gene fragment of canine distemper virus (CDV) by using the RT-PCR method with the subsequent sequencing of amplicons from total RNA isolated from 2 samples of Caspian seals, 15 samples of Baikal seals and from samples of dog's and sea-lion's brains. The above materials were phylogenetically analyzed. The heterogeneity of the virus circulating in the Baikal-seal population was demonstrated. Morbillivirus, that caused epizooty in Caspian seals, was shown to be a CDV variant, whose phosphoprotein gene structure was not different, within the analyzed stretch, from the corresponding gene of the most widespread variant of the Baikal seal virus. The data obtained suggest that morbillivirus could be transmitted by birds during their seasonal migrations.

摘要

采用逆转录聚合酶链反应（RT-PCR）方法，对来自里海海豹2份样本、贝加尔湖海豹15份样本以及狗和海狮大脑样本中分离出的总RNA扩增子进行测序，从而确定了犬瘟热病毒（CDV）磷蛋白基因片段的核苷酸序列。对上述材料进行了系统发育分析。结果表明，在贝加尔湖海豹种群中传播的病毒具有异质性。已证明，在里海海豹中引起 epizooty 的麻疹病毒是一种CDV变体，在分析的区域内，其磷蛋白基因结构与贝加尔湖海豹病毒最常见变体的相应基因没有差异。所获得的数据表明，麻疹病毒可能在鸟类季节性迁徙期间通过鸟类传播。