Elchuk S, Lucy C A, Burns K I
AECL Research, Chalk River Laboratories, Ontario, Canada.
Anal Chem. 1992 Oct 15;64(20):2339-43. doi: 10.1021/ac00044a007.
A procedure has been developed for measuring 147Pm in bioassay samples, based on the separation and preconcentration of 147Pm from the urine matrix by adsorption onto a conventional cation-exchange column with final separation and purification by HPLC using dynamic ion-exchange chromatography. The concentration of 147Pm is determined by collecting the appropriate HPLC fraction and measuring the 147Pm by liquid scintillation counting. The limit of detection is 0.1 Bq (3 fg) 147Pm based on a 500-mL sample of urine and a counting time of 30 min with a background of 100 cpm. Ten samples can be processed in 1.5-2 days.
已开发出一种用于测量生物测定样品中147Pm的方法，该方法基于通过吸附到传统阳离子交换柱上从尿液基质中分离和预浓缩147Pm，最后通过使用动态离子交换色谱的HPLC进行分离和纯化。通过收集适当的HPLC馏分并通过液体闪烁计数测量147Pm来确定其浓度。基于500 mL尿液样品、30分钟的计数时间和100 cpm的本底，检测限为0.1 Bq（3 fg）147Pm。十个样品可在1.5至2天内处理完毕。
