Aida Y, Okada K, Kageyama R, Amanuma H
Laboratory of Gene Technology and Safety, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Am J Vet Res. 1992 Nov;53(11):1988-91.
Tumor-associated antigens that are expressed in lymphosarcoma B cells of cattle with enzootic bovine leukosis had been analyzed in terms of their reactivity with 13 monoclonal antibodies (MAB). By use of flow cytometry and radioimmunoprecipitation, 1 of the MAB (c143) that recognized a tumor-associated antigen cross-reacted with blood lymphocytes (BL) from various mammalian species. By use of flow cytometry, the c143 MAB reacted with 10 to 49% of BL derived from human beings, mice, dogs, horses, pigs, llamas, sheep, goats, and cattle. Titer of the c143 MAB with BL from horses, pigs, human beings, and llamas ranged between 1:6.0 x 10(4) and 1:5.3 x 10(5); titer associated with BL of goats and sheep was 1:1.6 x 10(6); and that associated with BL of cattle was 1:4.3 x 10(7). The c143 MAB specifically immunoprecipitated 3 homologous proteins from cell extracts of caprine, ovine, and bovine BL (32-, 34-, and 36- to 37-kDa bovine proteins; 31-, 32-, and 36- to 37-kDa caprine proteins; and 31.5-, 33-, and 36- to 37-kDa ovine proteins), but none was immunoprecipitated from human, murine, canine, porcine, and llama BL. These results indicate that the avidity of the c143 MAB in binding to BL from ruminants (eg, goats, sheep, and cattle) is higher than that to BL from human beings, mice, dogs, horses, pigs, and llamas. In sheep, the c143 MAB could immunoprecipitate the aforementioned proteins from BL of the Suffolk breed, but not BL from the Corriedale breed, whereas the c143 MAB immunoprecipitated apparently identical proteins from BL of 4 breeds of cattle.
对患地方流行性牛白血病的牛淋巴肉瘤B细胞中表达的肿瘤相关抗原，已依据其与13种单克隆抗体（MAB）的反应性进行了分析。通过流式细胞术和放射免疫沉淀法，1种识别肿瘤相关抗原的MAB（c143）与来自各种哺乳动物物种的血液淋巴细胞（BL）发生交叉反应。通过流式细胞术，c143 MAB与来源于人类、小鼠、狗、马、猪、美洲驼、绵羊、山羊和牛的10%至49%的BL发生反应。c143 MAB与来自马、猪、人类和美洲驼的BL的效价在1:6.0×10⁴至1:5.3×10⁵之间；与山羊和绵羊的BL相关的效价为1:1.6×10⁶；与牛的BL相关的效价为1:4.3×10⁷。c143 MAB从山羊、绵羊和牛的BL细胞提取物中特异性免疫沉淀出3种同源蛋白（牛的32 kDa、34 kDa以及36至37 kDa蛋白；山羊的31 kDa、32 kDa以及36至37 kDa蛋白；绵羊的31.5 kDa、33 kDa以及36至37 kDa蛋白），但未从人类、小鼠、犬、猪和美洲驼的BL中免疫沉淀出任何蛋白。这些结果表明，c143 MAB与反刍动物（如山羊、绵羊和牛）的BL结合的亲和力高于与人类、小鼠、狗、马、猪和美洲驼的BL结合的亲和力。在绵羊中，c143 MAB可从萨福克品种的BL中免疫沉淀出上述蛋白，但不能从考力代品种的BL中免疫沉淀出上述蛋白，而c143 MAB从4个品种牛的BL中免疫沉淀出明显相同的蛋白。
