Koguchi A, Goryo M, Aida Y, Okada K
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Morioka, Japan.
Leukemia. 1997 Apr;11 Suppl 3:221-2.
A monoclonal antibody, (MAb) c143, that recognizes a tumor-associated antigen (TAA) that is upregulated on neoplastic B cells in cattle with enzootic bovine leukosis (EBL), was used as a marker to study disease progression. Immunohistochemical examination of neoplastic tissue and lymph nodes from animals with EBL, revealed three morphologically definable stages of change in the architecture of lymph nodes associated with infiltration and proliferation of neoplastic cells: 1) presence of c143 positive cells at the marginal sinus with no apparent changes in lymph node architecture at the earliest stage of neoplastic cell accumulation, 2) presence of positive cells extending into and distorting the architecture of the lymph node with clear evidence of proliferation, and 3) presence of positive cells throughout the lymph node with total disruption of lymph node architecture. The results indicated that, at the earliest stages of EBL, neoplastic cells in peripheral blood may accumulate at the marginal sinus area and subsequently proliferate and infiltrate pressing follicles leading to the development of clinical signs of lymphosarcoma.
一种单克隆抗体（MAb）c143，可识别一种肿瘤相关抗原（TAA），该抗原在患有地方流行性牛白血病（EBL）的牛的肿瘤性B细胞上上调，被用作研究疾病进展的标志物。对患有EBL的动物的肿瘤组织和淋巴结进行免疫组织化学检查，发现与肿瘤细胞浸润和增殖相关的淋巴结结构变化存在三个形态学上可定义的阶段：1）在肿瘤细胞积累的最早阶段，边缘窦处存在c143阳性细胞，淋巴结结构无明显变化；2）阳性细胞延伸至淋巴结并使其结构扭曲，有明显的增殖证据；3）阳性细胞遍布整个淋巴结，淋巴结结构完全破坏。结果表明，在EBL的最早阶段，外周血中的肿瘤细胞可能在边缘窦区域积累，随后增殖并浸润压迫滤泡，导致淋巴肉瘤临床症状的出现。
