Eversole L R, Miyasaki K T, Christensen R E
Section of Diagnostic Sciences, School of Dentistry, UCLA Center for the Health Sciences 90024.
Arch Oral Biol. 1992 Nov;37(11):963-8. doi: 10.1016/0003-9969(92)90068-j.
Calprotectin is a heterodimeric peptide isolated from neutrophil cytosol that exhibits profound antimicrobial effects. Using monoclonal antibody MAC 387, calprotectin was found to be expressed in oral keratinocytes from normal, non-inflamed oral mucosa. Orthokeratinized sites including the attached gingiva and hard palate expressed low levels of calprotectin with a restricted pattern; immunoreactants were identified only within subcorneal keratinocytes. Parakeratinized mucosa from the lips, soft palate, tongue and buccal mucosa expressed calprotectin in a more widespread, yet variable pattern, immunoreactants being detectable in only a portion of the spinous layer in some cases whereas in others the pattern of expression was more topographically diffuse. Antigen was not detected in basilar and lower strata cells. Both cytoplasmic and nuclear decoration could be identified. The results indicate that oral mucosa harbours an antimicrobial deterrent to micro-organisms that may enhance the physical epithelial barrier of host defence.
钙卫蛋白是一种从中性粒细胞胞质溶胶中分离出来的异二聚体肽，具有很强的抗菌作用。使用单克隆抗体MAC 387发现，在正常非炎症性口腔黏膜的口腔角质形成细胞中表达钙卫蛋白。包括附着龈和硬腭在内的正角化部位钙卫蛋白表达水平低且模式受限；免疫反应物仅在角膜下角质形成细胞内被识别。来自嘴唇、软腭、舌头和颊黏膜的不全角化黏膜以更广泛但可变的模式表达钙卫蛋白，在某些情况下，仅在棘层的一部分中可检测到免疫反应物，而在其他情况下，表达模式在地形上更弥散。在基底细胞和下层细胞中未检测到抗原。可识别细胞质和细胞核的装饰。结果表明，口腔黏膜含有一种对微生物的抗菌威慑物，可能会增强宿主防御的物理上皮屏障。
