Van Buskirk E M, Brett J
Invest Ophthalmol Vis Sci. 1978 Mar;17(3):258-71.
Facility of aqueous outflow and time-dependent changes in its hyaluronidase-sensitive and hyaluronidase-resistant components were evaluated in freshly excised canine eyes by constant pressure quantitative aqueous perfusion. Mean baseline facility of outflow was 0.24 microliter/min/mm Hg. With prolonged perfusion at constant intraocular pressure, facility of outflow was observed to increase almost linearly for at least 3 hr and continued to increase for up to 10 hr, reaching a maximum several times the initially measured facility. Perfusion with pooled dog aqueous humor did not prevent the time-dependent increase in measured facility. Rapid exchange of anterior chamber contents with perfusion solution alone produced an immediate threefold increase in facility, again followed by a gradual time-dependent facility increase. Rapid exchange of anterior chamber contents with hyaluronidase produced an immediate fivefold increase in facility with stabilization of measured facility over 3 hr and subsequent perfusion. The time-dependent changes in measured facility of outflow or "washout phenomenon" appeared to result from the gradual dissolution of the hyaluronidase-sensitive component of the barriers to aqueous outflow in the canine eye.
通过恒压定量房水灌注法，对新鲜摘除的犬眼房水流出功能及其对透明质酸酶敏感和不敏感成分随时间的变化进行了评估。平均基线流出功能为0.24微升/分钟/毫米汞柱。在恒定眼压下长时间灌注时，观察到流出功能至少在3小时内几乎呈线性增加，并持续增加长达10小时，达到最初测量值的数倍。用收集的犬房水灌注并不能阻止测量到的流出功能随时间增加。仅用灌注液快速更换前房内容物会使流出功能立即增加三倍，随后流出功能又会逐渐随时间增加。用透明质酸酶快速更换前房内容物会使流出功能立即增加五倍，在3小时及随后的灌注过程中测量到的流出功能保持稳定。测量到的流出功能随时间的变化或“冲洗现象”似乎是由于犬眼房水流出屏障中对透明质酸酶敏感成分的逐渐溶解所致。
