Pitman Martha B, Triratanachat Surang, Young Robert H, Oliva Esther
James Homer Wright Pathology Laboratories, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
Int J Gynecol Pathol. 2004 Jan;23(1):58-64. doi: 10.1097/01.pgp.0000101141.31270.a1.
Anti-hepatocyte antibody, hepatocyte paraffin 1, is a monoclonal antibody that is highly specific for normal and neoplastic hepatocytes and that can differentiate hepatocytic from nonhepatocytic tumors. This marker has been rarely studied in extra-hepatic tumors and to our knowledge has not been investigated in ovarian tumors with hepatoid differentiation. We studied hepatocyte paraffin 1 immunoreactivity in a series of ovarian hepatoid carcinomas, ovarian hepatoid yolk sac tumors (YSTs), and hepatocellular carcinomas metastatic to the ovary to assess the potential utility of hepatocyte paraffin 1 in differential diagnosis. Hepatocyte paraffin 1 positivity was seen in three of seven ovarian hepatoid carcinomas, five of eight hepatoid yolk sac tumors, and six of eight metastatic hepatocellular carcinomas. The extent of positivity ranged from <25% to >50% of the tumor cells. There was strong coarsely granular cytoplasmic staining in all three tumor types without a distinctive staining pattern in any group. The degree of hepatic differentiation correlated with hepatocyte paraffin 1 positivity in the three groups: 83% of the well differentiated tumors, 50% of the moderately differentiated tumors, and none of the poorly differentiated tumors were positive. All ovarian hepatoid carcinomas were either immunoreactive for alpha-fetoprotein or had an elevated serum alpha-fetoprotein level; more than half of these tumors were hepatocyte paraffin 1 negative. All but one hepatocyte paraffin 1 negative hepatoid yolk sac tumor and ovarian hepatocellular carcinoma were also negative for alpha-fetoprotein. In conclusion, hepatocyte paraffin 1 is positive in primary ovarian tumors with hepatoid differentiation, with the degree of hepatocyte paraffin 1 positivity correlating with the degree of hepatoid differentiation. Hepatocyte paraffin 1, however, is not useful in distinguishing metastatic hepatocellular carcinoma from primary ovarian hepatoid carcinoma or hepatoid yolk sac tumor.
抗肝细胞抗体（肝细胞石蜡1）是一种单克隆抗体，对正常和肿瘤性肝细胞具有高度特异性，能够区分肝细胞性肿瘤和非肝细胞性肿瘤。该标志物在肝外肿瘤中的研究较少，据我们所知，尚未在具有肝样分化的卵巢肿瘤中进行研究。我们研究了一系列卵巢肝样癌、卵巢肝样卵黄囊瘤（YSTs）以及转移至卵巢的肝细胞癌中肝细胞石蜡1的免疫反应性，以评估肝细胞石蜡1在鉴别诊断中的潜在用途。在7例卵巢肝样癌中有3例、8例肝样卵黄囊瘤中有5例以及8例转移性肝细胞癌中有6例检测到肝细胞石蜡1阳性。阳性范围为肿瘤细胞的<25%至>50%。所有三种肿瘤类型均有强烈的粗颗粒状细胞质染色，任何一组均无独特的染色模式。三组中肝样分化程度与肝细胞石蜡1阳性相关：高分化肿瘤中有83%、中分化肿瘤中有50%、低分化肿瘤中无一例呈阳性。所有卵巢肝样癌要么对甲胎蛋白免疫反应阳性，要么血清甲胎蛋白水平升高；这些肿瘤中超过一半肝细胞石蜡1阴性。除1例肝细胞石蜡1阴性的肝样卵黄囊瘤和卵巢肝细胞癌外，其余均对甲胎蛋白阴性。总之，肝细胞石蜡1在具有肝样分化的原发性卵巢肿瘤中呈阳性，肝细胞石蜡1阳性程度与肝样分化程度相关。然而，肝细胞石蜡1在鉴别转移性肝细胞癌与原发性卵巢肝样癌或肝样卵黄囊瘤方面并无帮助。
