García D, Leiro J, Delgado R, Sanmartín M L, Ubeira F M
Laboratorio de Farmacología, Centro de Química Farmacéutica, Ciudad de la Habana (Havana City), Cuba.
Phytother Res. 2003 Dec;17(10):1182-7. doi: 10.1002/ptr.1338.
The present study investigated the effects of orally administered Vimang (an aqueous extract of Mangifera indica) and mangiferin (the major polyphenol present in Vimang) on mouse antibody responses induced by inoculation with spores of microsporidian parasites. Inoculation induced specific antibody production with an exponential timecourse, peaking after about one month. Vimang significantly inhibited this antibody production from about three weeks post-inoculation, and most markedly by four weeks post-inoculation; by contrast, mangiferin had no significant effect. Determination of Ig isotypes showed that the IgM to IgG switch began about four weeks post-inoculation, with IgG2a predominating. Vimang significantly inhibited IgG production, but had no effect on IgM. Mangiferin did no affect either IgM or IgG2a, but significantly enhanced production of IgG1 and IgG2b. Neither Vimang nor mangiferin enhanced specific antibody secretion by splenic plasma cells from mice inoculated with microsporidian spores, whether administered in vivo before serum extraction or in vitro to the culture medium. Inoculation with spores induced splenomegaly, which was significantly reduced by Vimang and significantly enhanced by mangiferin. These results suggest that components of Mangifera indica extracts may be of potential value for modulating the humoral response in different immunopathological disorders.
本研究调查了口服Vimang（芒果水提取物）和芒果苷（Vimang中的主要多酚）对接种微孢子虫寄生虫孢子诱导的小鼠抗体反应的影响。接种诱导了特异性抗体的产生，其呈指数时间进程，在约一个月后达到峰值。Vimang从接种后约三周开始显著抑制这种抗体产生，在接种后四周时抑制最为明显；相比之下，芒果苷没有显著影响。Ig同种型的测定表明，IgM向IgG的转换在接种后约四周开始，以IgG2a为主。Vimang显著抑制IgG的产生，但对IgM没有影响。芒果苷对IgM或IgG2a均无影响，但显著增强了IgG1和IgG2b的产生。无论是在提取血清前体内给药还是在体外加入培养基中，Vimang和芒果苷均未增强接种微孢子虫孢子小鼠脾浆细胞的特异性抗体分泌。接种孢子诱导脾肿大，Vimang可使其显著减轻，而芒果苷则使其显著加重。这些结果表明，芒果提取物的成分可能对调节不同免疫病理疾病中的体液反应具有潜在价值。
