Taylor Adam L, Cottrell Garrison W, Kleinfeld David, Kristan William B
Department of Computer Science and Engineering, University of California, San Diego, La Jolla, California 92093, USA.
J Neurosci. 2003 Dec 10;23(36):11402-10. doi: 10.1523/JNEUROSCI.23-36-11402.2003.
In the leech, the command-like neuron called cell Tr2 is known to stop swimming, but the connections from cell Tr2 to the swim central pattern generator have not been identified. We used fluorescence resonance energy transfer voltage-sensitive dyes to identify three neurons that are synaptic targets of cell Tr2. We then used electrophysiological techniques to show that these connections are monosynaptic, chemical, and excitatory. Two of the novel targets, cell 256 and cell 54, terminate swimming when stimulated. These neurons are likely to mediate swim cessation caused by cell Tr2 activity, and thus play the role of intermediate control cells in the leech CNS.
在水蛭中,已知名为Tr2细胞的指令样神经元会停止游泳,但Tr2细胞与游泳中枢模式发生器之间的连接尚未明确。我们使用荧光共振能量转移电压敏感染料来识别作为Tr2细胞突触靶点的三个神经元。然后我们运用电生理技术表明这些连接是单突触、化学性且兴奋性的。两个新靶点,即256细胞和54细胞,受到刺激时会终止游泳。这些神经元可能介导由Tr2细胞活动引起的游泳停止,因此在水蛭中枢神经系统中起到中间控制细胞的作用。