Seki Motoaki, Satou Masakazu, Sakurai Tetsuya, Akiyama Kenji, Iida Kei, Ishida Junko, Nakajima Maiko, Enju Akiko, Narusaka Mari, Fujita Miki, Oono Youko, Kamei Ayako, Yamaguchi-Shinozaki Kazuko, Shinozaki Kazuo
Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
J Exp Bot. 2004 Jan;55(395):213-23. doi: 10.1093/jxb/erh007. Epub 2003 Dec 12.
Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones were isolated. The 3'-end expressed sequence tags (ESTs) of all 155,144 RAFL cDNAs were clustered into 14,668 non-redundant cDNA groups, about 60% of predicted genes. The sequence database of the RAFL cDNAs is useful for promoter analysis and the correct annotation of predicted transcription units and gene products. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. RAFL cDNA microarrays were prepared, containing independent full-length cDNA groups for analysing the expression profiles of genes under various stress- and hormone-treatment conditions and in various mutants and transgenic plants. In this review, recent progress on transcriptome analysis using the RAFL cDNA microarray is highlighted.
全长cDNA对于基因组序列的正确注释以及基因及其产物的功能分析至关重要。分离出了155,144个日本理化研究所拟南芥全长（RAFL）cDNA克隆。所有155,144个RAFL cDNA的3'端表达序列标签（EST）被聚类成14,668个非冗余cDNA组，约占预测基因的60%。RAFL cDNA的序列数据库可用于启动子分析以及预测转录单元和基因产物的正确注释。最近，已开发出cDNA微阵列分析用于对表达谱进行全局定量分析和同步分析。制备了RAFL cDNA微阵列，其包含独立的全长cDNA组，用于分析在各种胁迫和激素处理条件下以及在各种突变体和转基因植物中的基因表达谱。在本综述中，重点介绍了使用RAFL cDNA微阵列进行转录组分析的最新进展。
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