拟南芥全长cDNA文库的功能注释

Functional annotation of a full-length Arabidopsis cDNA collection.

作者信息

Seki Motoaki, Narusaka Mari, Kamiya Asako, Ishida Junko, Satou Masakazu, Sakurai Tetsuya, Nakajima Maiko, Enju Akiko, Akiyama Kenji, Oono Youko, Muramatsu Masami, Hayashizaki Yoshihide, Kawai Jun, Carninci Piero, Itoh Masayoshi, Ishii Yoshiyuki, Arakawa Takahiro, Shibata Kazuhiro, Shinagawa Akira, Shinozaki Kazuo

机构信息

Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center (GSC), 3-1-1 Koyadai, Tsukuba 305-0074, Japan.

出版信息

Science. 2002 Apr 5;296(5565):141-5. doi: 10.1126/science.1071006. Epub 2002 Mar 21.

DOI:10.1126/science.1071006

PMID:11910074

Abstract

Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.

摘要

全长互补DNA（cDNA）对于基因组序列的正确注释以及基因及其产物的功能分析至关重要。我们分离出了155,144个理化学研究所拟南芥全长（RAFL）cDNA克隆。155,144个RAFL cDNA的3'端表达序列标签（EST）被聚类为14,668个非冗余cDNA组，约占预测基因的60%。我们还从14,034个非冗余cDNA组中获得了5' EST，并构建了一个启动子数据库。RAFL cDNA的序列数据库对于启动子分析以及预测转录单元和基因产物的正确注释很有用。此外，全长cDNA是分析植物蛋白质表达谱、功能和结构的有用资源。