Hanano Shigeru, Tomatsu Hajime, Ohnishi Ai, Kobayashi Koichi, Kondo Yuki, Betsuyaku Shigeyuki, Takita Eiji, Ogata Yoshiyuki, Ozawa Keishi, Suda Kunihiro, Hosouchi Tsutomu, Nagase Takahiro, Suzuki Hideyuki, Sakurai Nozomu, Masumoto Hiroshi, Fukuda Hiroo, Shibata Daisuke
Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.
Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.
iScience. 2020 Jul 24;23(7):101332. doi: 10.1016/j.isci.2020.101332. Epub 2020 Jul 14.
Somatic plant cells can regenerate shoots and/or roots or adventitious embryonic calluses, which may induce organ formation under certain conditions. Such regenerations occur via dedifferentiation of somatic cells, induction of organs, and their subsequent outgrowth. Despite recent advances in understanding of plant regeneration, many details of shoot induction remain unclear. Here, we artificially induced shoot stem-like green organs (SSOs) in Arabidopsis thaliana roots via simultaneous induction of two transcription factors (TFs), ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 25 (ATHB25, At5g65410) and the B3 family transcription factor REPRODUCTIVE MERISTEM 7 (REM7, At3g18960). The SSOs exhibited negative gravitropism and differentiated vascular bundle phenotypes. The ATHB25/REM7 induced the expression of genes controlling shoot stem characteristics by ectopic expression in roots. Intriguingly, the restoration of root growth was seen in the consecutive and adjacent parts of the SSOs under gene induction conditions. Our findings thus provide insights into the development and regeneration of plant shoot stems.
植物体细胞能够再生出芽和/或根或不定胚性愈伤组织，在某些条件下这些组织可能诱导器官形成。此类再生过程通过体细胞去分化、器官诱导及其后续生长而发生。尽管在植物再生的理解方面取得了最新进展，但芽诱导的许多细节仍不清楚。在这里，我们通过同时诱导两个转录因子（TFs），即拟南芥同源盒蛋白25（ATHB25，At5g65410）和B3家族转录因子生殖分生组织7（REM7，At3g18960），在拟南芥根中人工诱导出茎状绿色器官（SSOs）。这些SSOs表现出负向重力性和分化的维管束表型。ATHB25/REM7通过在根中的异位表达诱导了控制茎特征的基因的表达。有趣的是，在基因诱导条件下，在SSOs连续且相邻的部分观察到根生长的恢复。因此，我们的研究结果为植物茎的发育和再生提供了见解。
