Reisdorf C, Malliavin T E, Delsuc M A
DCSO, Ecole Polytechnique, Palaiseau, France.
Biochimie. 1992 Sep-Oct;74(9-10):809-13. doi: 10.1016/0300-9084(92)90063-k.
Recently a method was proposed which permits the extraction of the exact interatomic distance information from the measurement of the evolution of a single cross-peak relative to the mixing time in a NOESY experiment. This is performed through a careful multi-exponential analysis allowing the extraction of the relaxation parameter, and, consequently, the inter-proton distance. We investigate in the present paper whether this technique, already evaluated theoretically, can be used in a real experimental case. We have recorded and analyzed a set of 56 NOESY experiments on a lysozyme sample. Some 81 nOe build-up curves obtained from these data were analyzed in terms of distance. It is shown that the correlation between the measured distances and the reference distances obtained from crystallographic studies, is quite good. An accuracy of the order of 10% is obtained.
最近有人提出了一种方法，该方法能够通过在NOESY实验中测量单个交叉峰相对于混合时间的演化来提取精确的原子间距离信息。这是通过仔细的多指数分析来实现的，该分析允许提取弛豫参数，进而得到质子间距离。在本文中，我们研究了这种已经在理论上得到评估的技术是否可以用于实际的实验情况。我们记录并分析了一组针对溶菌酶样品的56次NOESY实验。从这些数据中获得了约81条nOe积累曲线，并对其进行了距离分析。结果表明，测量距离与通过晶体学研究获得的参考距离之间的相关性相当好。获得了约10%的精度。
