Lu Xin-liang, Qian Ke-da, Tang Xun-qiu, Zhu Yong-liang
Department of Gastroenterology, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
J Zhejiang Univ Sci. 2004 Feb;5(2):242-5. doi: 10.1007/BF02840931.
To investigate the distribution of H.pylori antigens in the gastric mucosa in patients with H.pylori infection, and the relationship between the distribution and gastric cancer.
Of 112 patients confirmed by pathological study to have chronic superficial gastritis, precancerous changes (chronic atrophic gastritis, intestinal metaplasia or atypical hyperplasia) and gastric cancer, 28 were H.pylori negative and 84 were H.pylori positive. H.pylori antigens in the gastric mucosa were detected by immunohistochemistry.
The H.pylori positive group, comprised 12 of 22 (50.0%) in the chronic superficial gastritis group, 22 of 25 (88.0%) in the precancerous changes group and 13 of 35 (37.1%) in the gastric cancer group. The positive rates of H.pylori antigens in the cytoplasm progressively increased, respectively at 0.0% (0/12), 63.6% (14/22) and 84.6% (11/13) for the same groups (chi(2)=19.76, P=0.000); H.pylori antigens were located in the mucus layer and above the neck of the mucosal gland in 9 of 12 (75.0%) cases with chronic superficial gastritis, at the neck of the mucosal gland and the isthmus in 12 of 22 (54.5%) cases with precancerous changes, below the isthmus in 9 of 13 (69.2%) cases with gastric cancer (chi(2)=25.30, P=0.000). In the H.pylori negative group, no H.pylori antigen was observed.
With the progression of chronic superficial gastritis-->precancerous changes-->gastric cancer, H.pylori antigens progressively migrated from the outer part to the inner part of the cell, and from the superficial to the deep gastric mucosa.
探讨幽门螺杆菌（H.pylori）感染患者胃黏膜中H.pylori抗原的分布情况，以及该分布与胃癌的关系。
选取经病理研究确诊为慢性浅表性胃炎、癌前病变（慢性萎缩性胃炎、肠化生或异型增生）及胃癌的112例患者，其中28例H.pylori阴性，84例H.pylori阳性。采用免疫组织化学法检测胃黏膜中的H.pylori抗原。
H.pylori阳性组中，慢性浅表性胃炎组22例中有12例（50.0%）阳性，癌前病变组25例中有22例（88.0%）阳性，胃癌组35例中有13例（37.1%）阳性。相同组中，细胞质中H.pylori抗原阳性率逐渐升高，分别为0.0%（0/12）、63.6%（14/22）和84.6%（11/13）（χ²=19.76，P=0.000）；慢性浅表性胃炎组12例中有9例（75.0%）H.pylori抗原位于黏液层及黏膜腺颈部上方，癌前病变组22例中有12例（54.5%）位于黏膜腺颈部及峡部，胃癌组13例中有9例（69.2%）位于峡部以下（χ²=25.30，P=0.000）。H.pylori阴性组未观察到H.pylori抗原。
随着慢性浅表性胃炎→癌前病变→胃癌的进展，H.pylori抗原逐渐从细胞外部向内部迁移，从胃黏膜浅层向深层迁移。
