Breschi Lorenzo, Gobbi Pietro, Chersoni Stefano, Mazzotti Giovanni, Prati Carlo
Clinica Odontostomatologica, Department of Surgical Sciences, University of Trieste, Trieste, Italy.
Am J Dent. 2003 Sep;16 Spec No:77A-81A.
To evaluate if different acid treatments of dentin surface might remove different amount of mineralized dentin, thus exposing and modifying the CF network.
Dentin disks prepared from human third molars with a low-speed diamond saw were etched for 15 seconds with the tested acids: citric acid 10%, maleic acid 10%, 2.5% oxalic acid, 35% phosphoric acid and 24% EDTA gel. Specimens were then submitted to a 5% solution of NaOCl for 2 minutes or 5 minutes, fixed, and observed utilizing a field emission in lens SEM (FEISEM). Control specimens were also prepared by omitting the etching agent and/or the NaOCl solution.
The different acid treatments created specific dentin morphological pattern. CF exposure was in relationship with the acid used. The NaOCl solution greatly affects the acid etched dentin by removing the CF, nevertheless the effect of NaOCl was greatly influenced by the previous acid treatment.
评估牙本质表面不同的酸处理是否会去除不同量的矿化牙本质，从而暴露并改变胶原纤维网络。
用低速金刚石锯从人类第三磨牙制备牙本质片，用以下测试酸蚀刻15秒：10%柠檬酸、10%马来酸、2.5%草酸、35%磷酸和24%乙二胺四乙酸凝胶。然后将标本置于5%次氯酸钠溶液中2分钟或5分钟，固定后，利用场发射透镜扫描电子显微镜（FEISEM）观察。对照标本也通过省略蚀刻剂和/或次氯酸钠溶液来制备。
不同的酸处理产生了特定的牙本质形态模式。胶原纤维暴露与所用酸有关。次氯酸钠溶液通过去除胶原纤维极大地影响酸蚀刻的牙本质，然而次氯酸钠的效果受到先前酸处理的极大影响。
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