MICA基因多态性与口腔黏膜下纤维化风险

Polymorphism of the MICA gene and risk for oral submucous fibrosis.

作者信息

Liu Chung-Ji, Lee Yann-Jinn, Chang Kuo-Wei, Shih Yi-Ning, Liu Hsin-Fu, Dang Ching-Wen

机构信息

Departments of Oral and maxillofacial surgery, Taipei Mackay Memorial Hospital, School of Dentistry, National Yang-Ming University, Taipei, Taiwan.

出版信息

J Oral Pathol Med. 2004 Jan;33(1):1-6. doi: 10.1111/j.1600-0714.2004.00047.x.

DOI:10.1111/j.1600-0714.2004.00047.x

PMID:14675133

Abstract

BACKGROUND

Oral submucous fibrosis (OSF), an insidious, pre-cancerous, chronic disease that may affect the entire oral cavity and sometimes extend to the pharynx has been reported to be associated with immune function. The major histocompatibility complex (MHC) class I chain-related gene A (MICA) is expressed by keratinocytes and other epithelial cells, and its encoded protein interacts with gamma/delta T-cells localized in the submucosa. The MICA gene has a triplet repeat (GCT) polymorphism in the transmembrane domain resulting in five distinct allelic patterns.

METHODS

We analyzed MICA polymorphism in 80 OSF patients and 351 randomly selected unrelated controls by using the ABI Prism 377-18 DNA sequencer (Applied Biosystems) to analyze the sample DNA PCR products. The number of microsatellite repeats was estimated with Genescan 672 software (Applied Biosystems) with a standard size marker of GS-350 TAMRA.

RESULTS

The phenotype frequency of allele A6 of MICA in subjects with OSF was significantly higher than that in controls (OR = 3.48; 95% CI = 1.8-6.71; P = 0.0002), as was the frequency of the allele (OR = 2.65; 95% CI = 1.44-4.86; P = 0.001).

CONCLUSION

The results suggest that allele A6 in MICA might confer a risk for OSF.

摘要

背景

口腔黏膜下纤维化（OSF）是一种隐匿性的癌前慢性疾病，可累及整个口腔，有时还会延伸至咽部，据报道其与免疫功能有关。主要组织相容性复合体（MHC）I类链相关基因A（MICA）由角质形成细胞和其他上皮细胞表达，其编码的蛋白与位于黏膜下层的γ/δT细胞相互作用。MICA基因在跨膜结构域存在三联体重复序列（GCT）多态性，导致五种不同的等位基因模式。

方法

我们使用ABI Prism 377 - 18 DNA测序仪（应用生物系统公司）对80例OSF患者和351例随机选取的无关对照进行MICA多态性分析，以分析样本DNA的PCR产物。使用Genescan 672软件（应用生物系统公司）和GS - 350 TAMRA标准大小标记物估计微卫星重复序列的数量。