Sotto Mírian N, De Brito T, Silva Ana Maria G, Vidal Monica, Castro L G Martins
Department of Dermatology, Institute of Tropical Medicine, University of São Paulo Medical School, Av. Dr Enéas de Carvalho Aguiar 470, CEP 05403-000 São Paulo, Brazil.
J Cutan Pathol. 2004 Jan;31(1):14-8. doi: 10.1046/j.0303-6987.2004.0131.x.
Chromoblastomycosis is a chronic, suppurative, granulomatous mycosis usually confined to skin and subcutaneous tissues. The host defense mechanisms in chromoblastomycosis have not been extensively investigated. The purpose of the present study was to determine the distribution and pathways of the fungal antigen(s) and the possible role of the different immunocompetent cells in antigen processing in skin lesions.
The distribution of Fonsecaea pedrosoi antigen(s) in human skin was studied in 18 biopsies from 14 patients with chromoblastomycosis. A purified polyclonal immune serum raised in rabbits against metabolic antigen(s) of F. pedrosoi was used to detect yeast antigen(s) by immunohistochemical procedures. Double immunolabeling was performed with yeast antigen(s) and Langerhans' cells [labeled with anti-S100 protein monoclonal antibody (MoAb)], yeast antigen(s) and factor XIIIa+ dermal dendrocytes (immunolabeled with anti-factor XIIIa polyclonal antibody), and yeast antigen(s) and macrophages (labeled with CD 68 monoclonal antibody).
The F. pedrosoi antigen(s) accumulated in the skin macrophages and, in a few instances, in factor XIIIa+ dendrocytes and Langerhans' cells.
The data obtained suggest that chiefly macrophages, also Langerhans' cells and factor XIIIa+ dermal dendrocytes, function as antigen-presenting cells in chromoblastomycosis.
着色芽生菌病是一种慢性、化脓性、肉芽肿性真菌病，通常局限于皮肤和皮下组织。着色芽生菌病中的宿主防御机制尚未得到广泛研究。本研究的目的是确定真菌抗原的分布和途径，以及不同免疫活性细胞在皮肤病变抗原处理中的可能作用。
对14例着色芽生菌病患者的18份活检组织进行研究，以检测裴氏瓶霉抗原在人皮肤中的分布。用兔制备的针对裴氏瓶霉代谢抗原的纯化多克隆免疫血清，通过免疫组织化学方法检测酵母抗原。对酵母抗原与朗格汉斯细胞（用抗S100蛋白单克隆抗体标记）、酵母抗原与ⅩⅢa因子阳性真皮树突状细胞（用抗ⅩⅢa因子多克隆抗体免疫标记）以及酵母抗原与巨噬细胞（用CD68单克隆抗体标记）进行双重免疫标记。
裴氏瓶霉抗原积聚在皮肤巨噬细胞中，少数情况下也积聚在ⅩⅢa因子阳性树突状细胞和朗格汉斯细胞中。
所获得的数据表明，在着色芽生菌病中，主要是巨噬细胞，还有朗格汉斯细胞和ⅩⅢa因子阳性真皮树突状细胞，作为抗原呈递细胞发挥作用。
