Laboratory of Mycology, Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Scienses, Universidade de Sao Paulo, São Paulo, Brazil.
Mycopathologia. 2020 Apr;185(2):245-256. doi: 10.1007/s11046-020-00429-w. Epub 2020 Feb 1.
The chromoblastomycosis is a subcutaneous mycosis with a high morbidity rate, Fonsecaea pedrosoi being the largest etiologic agent of this mycosis, usually confined to the skin and subcutaneous tissues. Rarely people get the cure, because the therapies shown to be deficient and few studies report the host-parasite relationship. Dendritic cells (DCs) are specialized in presenting antigens to naïve T lymphocytes inducing primary immune responses. Therefore, we propose to study the migratory capacity of DCs after infection with conidia of F. pedrosoi. The phenotype of DCs was evaluated using cells obtained from footpad and lymph nodes of BALB/c mice after 12, 24 and 72 h of infection. After 24 and 72 h of infection, we found a significant decrease in DCs in footpad and a significant increase in the lymph nodes after 72 h. The expression of surface markers and co-stimulatory molecules were reduced in cells obtained from footpad. To better assess the migratory capacity of DCs migration from footpad, CFSE-stained conidia were injected subcutaneously. We found that after 12 and 72 h, CD11c cells were increased in regional lymph nodes, leading us to believe that DCs (CD11c) were able to phagocytic conidia present in footpad and migrated to regional lymph nodes.
着色芽生菌病是一种发病率较高的皮下真菌病，佩德罗索外瓶霉是这种真菌病的最大病原体，通常局限于皮肤和皮下组织。很少有人能完全治愈，因为现有的治疗方法显示效果不佳，而且很少有研究报告宿主-寄生虫关系。树突状细胞 (DC) 专门用于向幼稚 T 淋巴细胞呈递抗原，从而诱导初次免疫反应。因此，我们拟研究感染佩德罗索外瓶霉分生孢子后 DC 的迁移能力。通过感染后 12、24 和 72 小时从小鼠足垫和淋巴结中获得的细胞，评估 DC 的表型。感染后 24 和 72 小时，我们发现足垫中的 DC 明显减少，而 72 小时后淋巴结中的 DC 明显增加。从小鼠足垫中获得的细胞表面标记物和共刺激分子的表达减少。为了更好地评估 DC 的迁移能力，我们从足垫中注射了 CFSE 标记的分生孢子。我们发现，在 12 和 72 小时后，CD11c 细胞在区域淋巴结中增加，这使我们相信 DC（CD11c）能够吞噬足垫中存在的分生孢子并迁移到区域淋巴结。
