Burri Lena, Lithgow Trevor
Russell Grimwade School of Biochemistry & Molecular Biology, University of Melbourne, Parkville 3010, Australia.
Traffic. 2004 Jan;5(1):45-52. doi: 10.1046/j.1600-0854.2003.00151.x.
Trafficking of cargo molecules through the secretory pathway relies on packaging and delivery of membrane vesicles. These vesicles, laden with cargo, carry integral membrane proteins that can determine with which target membrane the vesicle might productively fuse. The membrane fusion process is highly conserved in all eukaryotes and the central components driving membrane fusion events involved in vesicle delivery to target membranes are a set of integral membrane proteins called SNAREs. The yeast Saccharomyces cerevisiae has served as an extremely useful model for characterizing components of membrane fusion through genetics, biochemistry and bioinformatics, and it is now likely that the complete set of SNAREs is at hand. Here, we present the details from the searches for SNAREs, summarize the domain structures of the complete set, review what is known about localization of SNAREs to discrete membranes, and highlight some of the surprises that have come from the search.
货物分子通过分泌途径运输依赖于膜泡的包装和递送。这些装载着货物的膜泡携带整合膜蛋白，这些蛋白能决定膜泡可能与哪个靶膜有效融合。膜融合过程在所有真核生物中高度保守，驱动膜泡递送至靶膜的膜融合事件的核心成分是一组称为SNARE的整合膜蛋白。酿酒酵母已成为通过遗传学、生物化学和生物信息学来表征膜融合成分的极其有用的模型，现在可能即将获得完整的SNARE蛋白集。在这里，我们展示了寻找SNARE蛋白的详细过程，总结了完整蛋白集的结构域结构，回顾了关于SNARE蛋白在离散膜上定位的已知信息，并强调了搜索过程中出现的一些意外发现。
