Cashman N R, Durham H D, Blusztajn J K, Oda K, Tabira T, Shaw I T, Dahrouge S, Antel J P
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Canada.
Dev Dyn. 1992 Jul;194(3):209-21. doi: 10.1002/aja.1001940306.
We have developed a series of mouse-mouse neural hybrid cell lines by fusing the aminopterin-sensitive neuroblastoma N18TG2 with motor neuron-enriched embryonic day 12-14 spinal cord cells. Of 30 neuroblastoma-spinal cord (NSC) hybrids displaying a multipolar neuron-like phenotype, 10 express choline acetyltransferase, and 4 induce twitching in cocultured mouse myotubules. NSC-19, NSC-34, and their subclones express additional properties expected of motor neurons, including generation of action potentials, expression of neurofilament triplet proteins, and acetylcholine synthesis, storage, and release. In addition, NSC-34 cells induce acetylcholine receptor clusters on cocultured myotubes, and undergo a vimentin-neurofilament switch with maturation in culture, similar to that occurring in neuronal development. NSC cell lines appear to model selected aspects of motor neuron development in an immortalized clonal system.
我们通过将对氨基蝶呤敏感的神经母细胞瘤N18TG2与富含运动神经元的胚胎第12至14天脊髓细胞融合，开发了一系列小鼠 - 小鼠神经杂交细胞系。在30个表现出多极神经元样表型的神经母细胞瘤 - 脊髓（NSC）杂交细胞中，10个表达胆碱乙酰转移酶，4个在共培养的小鼠肌管中诱导抽搐。NSC - 19、NSC - 34及其亚克隆表现出运动神经元预期的其他特性，包括动作电位的产生、神经丝三联体蛋白的表达以及乙酰胆碱的合成、储存和释放。此外，NSC - 34细胞在共培养的肌管上诱导乙酰胆碱受体簇的形成，并在培养过程中随着成熟经历波形蛋白 - 神经丝转换，类似于神经元发育过程中发生的情况。NSC细胞系似乎在一个永生化的克隆系统中模拟了运动神经元发育的某些方面。
