Haruki Hirohito, Gyurcsik Bela, Okuwaki Mitsuru, Nagata Kyosuke
Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.
FEBS Lett. 2003 Dec 18;555(3):521-7. doi: 10.1016/s0014-5793(03)01336-x.
The adenovirus (Ad) genome complexed with viral core proteins designated Ad core is the template for transcription of early genes and the first round of replication in Ad-infected cells. A cellular protein designated template-activating factor-I (TAF-I) is found to be involved in remodeling of the Ad core in vitro. Here we found that TAF-I interacts with the Ad DNA through core protein VII in infected cells in early phases of infection. In vitro binding assays using recombinant proteins showed that TAF-I forms ternary complexes with DNA-protein VII complexes.
与称为腺病毒核心(Ad core)的病毒核心蛋白复合的腺病毒(Ad)基因组是腺病毒感染细胞中早期基因转录和第一轮复制的模板。一种称为模板激活因子-I(TAF-I)的细胞蛋白被发现参与体外腺病毒核心的重塑。在这里,我们发现TAF-I在感染早期通过核心蛋白VII与感染细胞中的腺病毒DNA相互作用。使用重组蛋白的体外结合试验表明,TAF-I与DNA-蛋白VII复合物形成三元复合物。