Allicotti Gina, Borras Eva, Pinilla Clemencia
Torrey Pines Institute for Molecular Studies, San Diego, California 92121, USA.
J Immunoassay Immunochem. 2003;24(4):345-58. doi: 10.1081/IAS-120025772.
In an effort to improve the quantification of the low levels of cytokines released in response to antigenic stimulation of T cells, a sandwich dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA) was developed and compared to a standard sandwich ELISA. The DELFIA enhanced the sensitivity of a mouse IL-2 assay 8- to 27-fold, and a human GM-CSF assay 10-fold, as compared to colorimetric ELISA. The increase in sensitivity allows for the use of lower sample volumes per well, and the ability to run more assays per supernatant sample. This sensitive, nonisotopic alternative to other cytokine detection methods will be useful for those researchers wanting to quantitate low levels of antigen-driven cytokine production.
为了改进对T细胞抗原刺激后释放的低水平细胞因子的定量分析，开发了一种夹心解离增强镧系元素荧光免疫分析（DELFIA），并与标准夹心酶联免疫吸附测定（ELISA）进行比较。与比色ELISA相比，DELFIA将小鼠IL-2检测的灵敏度提高了8至27倍，将人GM-CSF检测的灵敏度提高了10倍。灵敏度的提高使得每孔可以使用更低的样品体积，并且每个上清液样品能够进行更多的检测。这种对其他细胞因子检测方法敏感的非同位素替代方法，对于那些想要定量低水平抗原驱动细胞因子产生的研究人员将是有用的。
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