Takahashi Masakazu, Tsujimoto Kazuhisa, Yamada Hideyuki, Takagi Hiroshi, Nakamori Shigeru
Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka-cho, Yoshida-gun, Fukui 910-1195, Japan.
Biotechnol Lett. 2003 Nov;25(21):1805-9. doi: 10.1023/a:1026284620236.
Sericin is the silk protein that covers fibroin fibers and functions as a 'glue' in the cocoons of silkworms, and its most abundant component, Ser1, contains repeats of Ser- and Thr-rich 38 amino acid residues. The viability of Sf9 insect cells was 20, 57 and 49% on the fifth day and 41, 91 and 70% on the ninth day after serum deprivation in the presence of no additives, 3000 microg sericin hydrolysate and 350 microg SerD (the peptide containing the two repetitive units) ml(-1), respectively. Thus, the sericin samples were useful in preventing cell death and promoting cellular growth after acute serum deprivation.
丝胶是覆盖在丝素纤维上的丝蛋白，在蚕茧中起到“胶水”的作用，其最丰富的成分Ser1含有富含丝氨酸和苏氨酸的38个氨基酸残基的重复序列。在无添加剂、添加3000微克丝胶水解产物和350微克SerD（含有两个重复单元的肽）每毫升的情况下，血清剥夺后第5天，Sf9昆虫细胞的活力分别为20%、57%和49%，第9天分别为41%、91%和70%。因此，丝胶样品在急性血清剥夺后可有效防止细胞死亡并促进细胞生长。
