Takahashi Masakazu, Tsujimoto Kazuhisa, Kato Youichi, Yamada Hideyuki, Takagi Hiroshi, Nakamori Shigeru
Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjojima, 910-1195 Fukui, Japan.
Biotechnol Lett. 2005 Jul;27(13):893-7. doi: 10.1007/s10529-005-7177-5.
Sericin is the silk protein enveloping fibroin fibers in cocoons. Sericin hydrolysate protects cultured Sf9 insect cells from death caused by serum deprivation; the activity depends on the repeats of 38 amino acids. A partial peptide from the 38 residues, SGGSSTYGYS, inhibited serum-deprivation death as well. Cell viabilities in the presence of 10% (v/v) foetal calf serum, no additives and 1 mM: SGGSSTYGYS were 96, 12 and 31% on the third day after inoculation, respectively. Aromatic residues seemed to be important because SGGSSTWGWS had the same activity as SGGSSTYGYS but SGGSSTAGAS had no activity.
丝胶是包裹茧中丝素纤维的丝蛋白。丝胶水解产物可保护培养的Sf9昆虫细胞免于血清剥夺导致的死亡；该活性取决于38个氨基酸的重复序列。来自38个残基的部分肽SGGSSTYGYS也能抑制血清剥夺诱导的死亡。接种后第三天，在含有10%（v/v）胎牛血清、无添加剂和1 mM SGGSSTYGYS的情况下，细胞活力分别为96%、12%和31%。芳香族残基似乎很重要，因为SGGSSTWGWS与SGGSSTYGYS具有相同的活性，但SGGSSTAGAS没有活性。
