[Effects of flavone from leaves of Diospyros kaki on adventitial fibroblast proliferation induced by advanced glycation end-products in vitro].

OBJECTIVE

To observe the effects of flavone from the leaves of Diospyros kaki on proliferation of adventitial fibroblasts induced by advanced glycation end-products (AGEs) in vitro.

METHODS

NIH/3T3 cells cultured in vitro were treated with both AGEs and flavone from the leaves of Diospyros Kaki for observation in comparison with the cells that received treatments with either AGEs or flavone, or neither. The ratio of cell proliferation was determined by non-radioactive MTS/PES assay.

RESULTS

The ratio of cell proliferation was 0.840+/-0.061 in the untreated control group, and was 1.330+/-0.055, 1.210+/-0.119, 1.029+/-0.076 and 0.792+/-0.060 in AGEs groups corresponding to AGE concentrations of 100, 50, 10 and 1 microg/ml respectively. AGEs significantly induced fibroblast proliferation in a dose-dependent manner when the concentration was above 10 microg/ml (P<0.05), as compared with the untreated control group. (P<0.05). The ratio of cell proliferation was 0.829+/-0.056 in cells treated with flavone at the concentration of 50 microg/ml, which alone failed to affect fibroblast proliferation (P>0.05). With AGEs stimulation, however, flavone from the leaves of Diospyros kaki significantly inhibited the proliferation of the fibroblasts (P<0.05).

CONCLUSION

Flavone from the leaves of Diospyros kaki can significantly inhibit the proliferation of adventitial fibroblasts stimulated by AGEs in vitro.