Recombinant Lapemis hardwickii phospholipase A2 inhibits rat vascular smooth muscle cell proliferation stimulated by advanced glycation end products.

OBJECTIVE

To observe the effects of recombinant Lapemis hardwickii phospholipase A(2) (rPLA(2)) on vascular smooth muscle cell (VSMC) proliferation stimulated by advanced glycation end products (AGEs).

METHODS

Rat aortic VSMCs were cultured in vitro and stimulated with AGEs of different concentrations prior to co-treatment with rPLA(2) and AGEs. The response of the VSMCs to these treatments was observed in comparison with that of the control group. Non-radioactive MTS/PES assay was adopted for the quantification of the cell proliferation ratio.

RESULTS

Compared with the control group, AGEs significantly stimulated VSMCs proliferation (P < 0.05), but even with AGEs stimulation, rPLA(2) inhibited the growth of VSMCs (0.753+/-0.098 vs 1.100+/-0.226, P < 0.05).

CONCLUSION

rPLA(2) can significantly inhibit the proliferation of VSMCs stimulated by AGEs.