Kircher Moritz F, Mahmood Umar, King Raymond S, Weissleder Ralph, Josephson Lee
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA.
Cancer Res. 2003 Dec 1;63(23):8122-5.
The determination of brain tumor margins both during the presurgical planning phase and during surgical resection has long been a challenging task in the therapy of brain tumor patients. Using a model of gliosarcoma with stably green fluorescence protein-expressing 9L glioma cells, we explored a multimodal (near-infrared fluorescent and magnetic) nanoparticle as a preoperative magnetic resonance imaging contrast agent and intraoperative optical probe. Key features of nanoparticle metabolism, namely intracellular sequestration by microglia and the combined optical and magnetic properties of the probe, allowed delineation of brain tumors both by preoperative magnetic resonance imaging and by intraoperative optical imaging. This prototypical multimodal nanoparticle has unique properties that may allow radiologists and neurosurgeons to see the same probe in the same cells and may offer a new approach for obtaining tumor margins.
在脑肿瘤患者的治疗中，无论是在术前规划阶段还是手术切除过程中，确定脑肿瘤边界一直是一项具有挑战性的任务。我们使用一种含有稳定表达绿色荧光蛋白的9L胶质瘤细胞的胶质肉瘤模型，探索了一种多模态（近红外荧光和磁性）纳米颗粒，作为术前磁共振成像造影剂和术中光学探针。纳米颗粒代谢的关键特征，即小胶质细胞对其的细胞内隔离以及探针的光学和磁性综合特性，使得通过术前磁共振成像和术中光学成像都能够勾勒出脑肿瘤。这种典型的多模态纳米颗粒具有独特的特性，可能使放射科医生和神经外科医生能够在同一细胞中看到相同的探针，并可能为获取肿瘤边界提供一种新方法。
