发育中和成年大鼠视神经中的牛磺酸、γ-氨基丁酸和胶质纤维酸性蛋白免疫反应性

Taurine, GABA and GFAP immunoreactivity in the developing and adult rat optic nerve.

作者信息

Lake N

机构信息

Department of Physiology, McGill University, Montreal, Que., Canada.

出版信息

Brain Res. 1992 Nov 20;596(1-2):124-32. doi: 10.1016/0006-8993(92)91539-q.

DOI:10.1016/0006-8993(92)91539-q

PMID:1467978

Abstract

The localizations of taurine, gamma-aminobutyric acid (GABA) and glial fibrillary acidic protein (GFAP) within the developing rat optic nerve were determined using immunocytochemical techniques on tissues from animals ranging in age from embryonic day 20 to postnatal 28 days. Mature nerves from 3-4-month-old adults were also examined. At the younger ages, taurine immunoreactivity was intense and localized specifically to the optic nerve axons, but by postnatal day 15 and thereafter its predominant localization was in macroglia. Some of these glia were astrocytes as indicated by the specific marker, GFAP. GABA immunoreactivity was present at the same time as taurine but was found only in macroglia. In mature nerves the patterns of taurine, GABA and GFAP distribution (within glia) were highly similar.

摘要

运用免疫细胞化学技术，对胚胎第20天至出生后28天大鼠的视神经组织进行检测，确定了牛磺酸、γ-氨基丁酸（GABA）和胶质纤维酸性蛋白（GFAP）在发育中的大鼠视神经内的定位。同时也对3 - 4月龄成年大鼠的成熟神经进行了检查。在较年幼时，牛磺酸免疫反应强烈且特异性定位于视神经轴突，但在出生后第15天及之后，其主要定位在大胶质细胞。如特异性标志物GFAP所示，其中一些胶质细胞是星形胶质细胞。GABA免疫反应与牛磺酸同时出现，但仅在大胶质细胞中发现。在成熟神经中，牛磺酸、GABA和GFAP（在胶质细胞内）的分布模式高度相似。