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埃及伊蚊(双翅目:蚊科)的遗传分化,巴西主要的登革热传播媒介。

Genetic differentiation of Aedes aegypti (Diptera: Culicidae), the major dengue vector in Brazil.

作者信息

Ayres C F J, Melo-Santos M A V, Solé-Cava A M, Furtado A F

机构信息

Departamento de Entomologia, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife-PE, Brazil.

出版信息

J Med Entomol. 2003 Jul;40(4):430-5. doi: 10.1603/0022-2585-40.4.430.

DOI:10.1603/0022-2585-40.4.430
PMID:14680106
Abstract

In 2000, Brazil reported 180,137 cases of dengue, approximately 80% of the total in the Americas. However, little is known about gene flow among the vector populations in Brazil. Random amplified polymorphic DNA (RAPD) was used to study the genetic structure of Aedes aegypti in 15 populations from five states, with a range extending 2,800 km. An analysis of 47 polymorphic RAPD loci estimated gene flow at the macro- (different states) and micro- (different cities) geographical levels. Genetic polymorphism was high (H(S) = 0.274), and high levels of genetic differentiation existed both between different states (G(ST) = 0.317) and between cities or neighborhoods in each state (G(ST) = 0.085-0.265). These values are higher than those described for any other populations of A. aegypti.

摘要

2000年,巴西报告了180137例登革热病例,约占美洲总数的80%。然而,对于巴西病媒种群之间的基因流动情况却知之甚少。随机扩增多态性DNA(RAPD)被用于研究来自五个州的15个种群中埃及伊蚊的遗传结构,其范围延伸达2800公里。对47个多态性RAPD位点的分析在宏观(不同州)和微观(不同城市)地理层面估计了基因流动情况。遗传多态性较高(H(S)=0.274),不同州之间(G(ST)=0.317)以及每个州内不同城市或街区之间(G(ST)=0.085 - 0.265)均存在高度的遗传分化。这些数值高于针对其他任何埃及伊蚊种群所描述的数值。

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