Szkanderová S, Vávrová J, Rézacová M, Vokurková D, Pavlová S, Smardová J, Stulík J
Institute of Radiobiology and Molecular Pathology, Purkyne Military Medical Academy, Hradec Králové, Czech Republic.
Folia Biol (Praha). 2003;49(5):191-6.
Exposure of human leukaemia MOLT-4 cells to ionizing irradiation led to apoptosis, which was detected by flow cytometric analysis and degradation of the nuclear lamina. The multiple signalling pathways triggered by either membrane or DNA damage play a critical role in radiation-induced apoptosis. The response to DNA damage is typically associated with the p53 protein accumulation. In this study, we proved that the transcriptionally active p53 variant occurs in the MOLT-4 cells and its abundance alteration is triggered in the gamma-irradiated cell population concomitantly with phosphorylation at both the serine-392 and serine-15 residues. The p21 upregulation followed the p53 phosphorylation process in irradiated MOLT-4 cells.
将人白血病MOLT-4细胞暴露于电离辐射会导致细胞凋亡，这可通过流式细胞术分析和核纤层降解来检测。由膜损伤或DNA损伤触发的多种信号通路在辐射诱导的细胞凋亡中起关键作用。对DNA损伤的反应通常与p53蛋白积累有关。在本研究中，我们证明转录活性p53变体存在于MOLT-4细胞中，并且在γ射线照射的细胞群体中，其丰度变化与丝氨酸392和丝氨酸15残基的磷酸化同时被触发。在受辐射的MOLT-4细胞中，p21上调跟随p53磷酸化过程。
