Liu Bing, Zhang Hong, Xie Yi, Hao Jifang, Duan Xin, Zhou Qingming, Qiu Rong, Zhou Guangming
Institute of Modern Physics, Chinese Academy of Sciences, Beijing 100039, China.
Eur J Gastroenterol Hepatol. 2007 Sep;19(9):749-54. doi: 10.1097/MEG.0b013e328220ebeb.
The purpose of this study is to investigate the effect of subclinical-dose C-beam irradiation on cell cycle and cell apoptosis in hepatocarcinoma cells.
The HepG2 cells were exposed to 0-2.0 Gy of either the C beam or a gamma-ray. Cell survival was detected by clonogenic assay. Cell cycle was determined by flow-cytometry analysis. The apoptosis was monitored by fluorescence microscope with DAPI staining. p53 and p21 expression were detected by Western blot.
The G0/G1 cells in the irradiated groups were significantly more than those in the control (P<0.05). The C-ion irradiation had a greater effect on the cell cycle of HepG2 cells (including promoting G1-phase and G2-phase arrest) than gamma-ray irradiation. The apoptotic cells induced by C beam were significantly more numerous than those induced by gamma-ray (P<0.05). The carbon ions had a stronger effect on p53 and p21 expression than the gamma-ray irradiation. The survival fractions for cells irradiated by C beam were significantly smaller than those irradiated by gamma-ray (P<0.05).
The subclinical-dose C-beam irradiation significantly suppresses HepG2 cells through cell-cycle arrests and cell apoptosis in contrast to same-dose gamma-ray irradiation.
本研究旨在探讨亚临床剂量碳离子束辐照对肝癌细胞细胞周期及细胞凋亡的影响。
将HepG2细胞分别暴露于0 - 2.0 Gy的碳离子束或γ射线。采用克隆形成试验检测细胞存活率。通过流式细胞术分析确定细胞周期。用DAPI染色，通过荧光显微镜监测细胞凋亡。采用蛋白质免疫印迹法检测p53和p21的表达。
辐照组的G0/G1期细胞显著多于对照组（P<0.05）。碳离子辐照对HepG2细胞的细胞周期影响更大（包括促进G1期和G2期阻滞），比γ射线辐照更明显。碳离子束诱导的凋亡细胞明显多于γ射线诱导的凋亡细胞（P<0.05）。碳离子对p53和p21表达的影响比γ射线辐照更强。碳离子束辐照的细胞存活分数显著低于γ射线辐照的细胞（P<0.05）。
与相同剂量的γ射线辐照相比，亚临床剂量碳离子束辐照通过细胞周期阻滞和细胞凋亡显著抑制HepG2细胞。
