Wang Wen-xia, Sun Shan-zhen, Yang Pi-shan, Ma Bo-long, Long Yan
Department of Periodontics and Mucosal Diseases, College of Stomatology, Shandong University, Jinan 250012, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2003 Sep;38(5):361-3.
To investigate the expression and alteration (including homozygous deletion and mutation) of MTS1 gene in precancerous lesions and squamous cell carcinomas (SCC) of oral mucosa, and to analyse the function of MTS1 gene alteration in oral mucosal carcinogenesis.
The expression of p16 protein produced by MTS1 gene was examined with immunohistochemical SP method in 10 normal oral mucosas, 30 precancerous lesions (10 mild, 10 moderate and 10 severe dysplasia respectively) and 45 squamous cell carcinomas (SCCI18, SCCII 19, SCCIII 8). The deletion and mutation of exon1 and exon2 of MTS1 gene were examined with methods of PCR and SSCP in these same samples.
All the precancerous lesions had p16 protein expression and no alteration of MTS1 gene. In SCC, the positive rate of p16 protein was 60.0% with 72.2% in SCCI, 57.9% in SCCII, 37.5% in SCC III, and there were no significant difference among the three groups by chi2 test (P>0.05). Gene homozygous deletion of exon1 and/or exon2 was detected in 10 cases, and gene mutation in 4 cases. The whole rate of gene alteration was 31.1% (14/45). The MTS1 gene alteration rate was 27.8% in SCCI, 31.6% in SCCII, 37.5% in SCC III and there was also no significant difference among the three groups by chi2 test (P>0.05). In SCC with local lymph nodes metastasis, MTS1 alteration rate was 57.1%, while in SCC with no lymph nodes metastasis was 8.3%, and there was significant difference by chi2 test (P<0.05).
MTS1 gene alteration is not an early event in the carcinogenesis of oral mucosa and can not be used as a biology mark to examine oral precancerous lesions. MTS1 gene may play a certain role in the progression of oral squamous cell carcinomas.
研究MTS1基因在口腔黏膜癌前病变及鳞状细胞癌（SCC）中的表达及改变（包括纯合缺失和突变），并分析MTS1基因改变在口腔黏膜癌变中的作用。
采用免疫组织化学SP法检测10例正常口腔黏膜、30例癌前病变（分别为10例轻度、10例中度和10例重度发育异常）及45例鳞状细胞癌（SCCI 18例、SCCII 19例、SCCIII 8例）中MTS1基因所产生的p16蛋白的表达。采用PCR和SSCP方法检测上述相同样本中MTS1基因外显子1和外显子2的缺失和突变。
所有癌前病变均有p16蛋白表达，且MTS1基因无改变。在SCC中，p16蛋白阳性率为60.0%，其中SCCI中为72.2%，SCCII中为57.9%，SCCIII中为37.5%，经χ2检验三组间差异无统计学意义（P>0.05）。检测到10例存在外显子1和/或外显子2基因纯合缺失，4例存在基因突变。基因改变总发生率为31.1%（14/45）。MTS1基因改变率在SCCI中为27.8%，SCCII中为31.6%，SCCIII中为37.5%，经χ2检验三组间差异无统计学意义（P>0.05）。在有局部淋巴结转移的SCC中，MTS1改变率为57.1%，而在无淋巴结转移的SCC中为8.3%，经χ2检验差异有统计学意义（P<0.05）。
MTS1基因改变不是口腔黏膜癌变的早期事件，不能作为检测口腔癌前病变的生物学标志物。MTS1基因可能在口腔鳞状细胞癌的进展中起一定作用。
