Buajeeb Waranun, Poomsawat Sopee, Punyasingh Jirapa, Sanguansin Sirima
Department of Oral Medicine, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
J Oral Pathol Med. 2009 Jan;38(1):104-8. doi: 10.1111/j.1600-0714.2008.00710.x.
Expression of p16 has been proposed as a marker for malignant transformation. This study aimed to evaluate p16 expression in oral squamous cell carcinoma (OSCC) and premalignant lesions including oral leukoplakia (OL) with and without dysplasia.
Expression of p16 was investigated in 56 samples including OSCC, OL with and without dysplasia, and normal oral mucosa. Expression of p16 was identified by immunohistochemistry, using the CINtecTM p16INK4a Histology Kit. Both nuclear and/or cytoplasmic staining of the keratinocytes were considered to be positive and the percentage of positive cells was calculated.
Expression of p16 was detected in 3/16 (18.75%) cases of OSCC, in 4/15 (26.7%) cases of OL without dysplasia, and in none of OL with dysplasia and normal mucosa. No significant differences in p16 expression prevalence were found among OSCC, OL with and without dysplasia and normal mucosa. The percentages of positive cells in OSCC and OL without dysplasia were 0.89 and 0.17, respectively. No significant difference in the percentage of positive keratinocytes was found.
As a marker, p16 is not reliable for oral mucosal dysplasia and malignant transformation.
p16的表达已被提议作为恶性转化的标志物。本研究旨在评估p16在口腔鳞状细胞癌（OSCC）及癌前病变（包括有或无发育异常的口腔白斑（OL））中的表达情况。
对56份样本进行p16表达情况的研究，这些样本包括OSCC、有或无发育异常的OL以及正常口腔黏膜。使用CINtecTM p16INK4a组织学试剂盒通过免疫组织化学法鉴定p16的表达。角质形成细胞的细胞核和/或细胞质染色均被视为阳性，并计算阳性细胞的百分比。
在16例OSCC病例中有3例（18.75%）检测到p16表达，在15例无发育异常的OL病例中有4例（26.7%）检测到p16表达，而在有发育异常的OL和正常黏膜中均未检测到。在OSCC、有或无发育异常的OL以及正常黏膜之间，p16表达率未发现显著差异。OSCC和无发育异常的OL中阳性细胞的百分比分别为0.89和0.17。阳性角质形成细胞的百分比未发现显著差异。
作为一种标志物，p16对于口腔黏膜发育异常和恶性转化并不可靠。
