Leng Shu-Guang, Zheng Yu-Xin, Dai Yu-Fei, Niu Yong, Zhang Wen-Zhong, Wang Ya-Wen, Li Xiao-Hua, Pan Zu-Fei, Xiao Jun, Wang Zhong-Xu, Li Tao, He Feng-Sheng
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2003 Sep;37(5):327-30.
To investigate the relationship between the urinary 1-hydroxypyrene level and cytokinesis-block micronucleus and the olive moment of comet assay in peripheral blood lymphocyte in coke oven workers.
One hundred and thirty-three workers from a coke plant and 28 referents without occupational PAH exposure were recruited in this study. Urinary level of 1-hydroxypyrene was measured by alkaline hydrolysis combined with high performance liquid chromatography as an internal exposure dose, and the DNA and chromosomal damage of peripheral blood lymphocyte were evaluated with comet assay and cytokinesis-block micronucleus method. Personal information including occupational history, age, sex, smoking and alcohol drinking, was collected by questionnaire.
There existed a good correlationship between the urinary level of 1-hydroxypyrene and frequency of micronuclei per 1 000 binucleated cells or the olive moment of comet assay in the study subjects, after adjusting for sex, age, smoking and alcohol drinking (r > 0.25, P < 0.01). One hundred and sixty-one subjects were divided into three groups by their urine 1-hydroxypyrene level (expressed as 0.30 - 2.44, 2.45 - 7.09 and 7.10 - 33.10 micro mol/mol Cr), and the geometric means of their urinary levels of 1-hydroxypyrene were 1.14, 4.32 and 12.49 micro mol/mol Cr, respectively. After adjusting for age, sex, smoking and alcohol drinking by multiple nonparametric analysis of covariance, the median of olive moment of comet assay in the group of 7.10 - 33.10 micro mol/mol Cr was 3.67, significantly higher than that in the groups of 0.30 - 2.44 and 2.45 - 7.09; and the micronuclei frequencies in the groups of 2.45 - 7.09 and 7.10 - 33.10 micro mol/mol Cr were 8.00 per thousand and 7.50 per thousand, respectively, significantly higher than that in the group of 0.30 - 2.44 micro mol/mol Cr (6.00 per thousand ).
The comet assay of peripheral blood lymphocyte was more suitable to detect the PAHs-induced early genotoxicity, than the cytokinesis-block micronucleus.
探讨焦炉工人尿中1-羟基芘水平与外周血淋巴细胞胞质分裂阻滞微核及彗星试验橄榄尾矩之间的关系。
本研究招募了某焦化厂的133名工人和28名无职业性多环芳烃暴露的对照者。采用碱性水解结合高效液相色谱法测定尿中1-羟基芘水平作为内暴露剂量，并用彗星试验和胞质分裂阻滞微核法评估外周血淋巴细胞的DNA和染色体损伤。通过问卷调查收集包括职业史、年龄、性别、吸烟和饮酒等个人信息。
在对性别、年龄、吸烟和饮酒进行校正后，研究对象尿中1-羟基芘水平与每1000个双核细胞的微核频率或彗星试验的橄榄尾矩之间存在良好的相关性（r>0.25，P<0.01）。161名受试者按尿中1-羟基芘水平（表示为0.30 - 2.44、2.45 - 7.09和7.10 - 33.10微摩尔/摩尔肌酐）分为三组，其尿中1-羟基芘水平的几何均值分别为1.14、4.32和12.49微摩尔/摩尔肌酐。经多因素非参数协方差分析对年龄、性别、吸烟和饮酒进行校正后，7.10 - 33.10微摩尔/摩尔肌酐组彗星试验橄榄尾矩的中位数为3.67，显著高于0.30 - 2.44和2.45 - 7.09组；2.45 - 7.09和7.10 - 33.10微摩尔/摩尔肌酐组的微核频率分别为千分之8.00和千分之7.50，显著高于0.30 - 2.44微摩尔/摩尔肌酐组（千分之6.00）。
外周血淋巴细胞彗星试验比胞质分裂阻滞微核试验更适合检测多环芳烃诱导的早期遗传毒性。
