叶绿酸对反式苯并(a)芘-反式-7,8-二氢二醇-9,10-环氧化物的抗转化活性

[Antitransforming activity of chlorophyllin against trans-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide].

作者信息

Ji Wei-Dong, Jiang Yi-Guo, Wang Min, Feng Su-Mei

机构信息

Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou 510182, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2003 Sep;37(5):335-7.

PMID:14680595

Abstract

OBJECTIVE

To study the inhibitory effect of chlorophyllin (CHL) on trans-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (BPDE) induced malignant transformation in human bronchial epithelial cell line (16HBE).

METHODS

10, 50 or 100 micro mol/L CHL were added into the media during the cells transformation induced by BPDE, and the malignant degree of transformed cells were identified by the ConA agglutination test and the assay for anchorage-independent growth and tumorigenicity.

RESULTS

After the cells were cultured for 25 times, the time of cells agglutination in groups treated with both CHL and BPDE was increased significantly; the colony formation efficiency in soft agar in groups treated with both CHL and BPDE (7.4 per thousand, 11.4 per thousand and 14.4 per thousand ) showed significant decrease (P < 0.05) in dose-dependent manner, as compared with that in group treated with BPDE alone (19.6 per thousand ). Cells treated with both CHL and BPDE or BPDE alone developed tumor in nude mice, a squamous carcinoma confirmed by histopathological examination. The volume of tumor in groups treated with both CHL and BPDE (0.43 +/- 0.13) cm(2), (0.22 +/- 0.04) cm(2) and (0.10 +/- 0.06) cm(3) was significantly smaller (P < 0.05) and dose-dependent, as compared with that in the group treated with BPDE alone (1.71 +/- 0.37) cm(3).

CONCLUSION

CHL showed significant antitransforming ability in human bronchial epithelial cell line induced by BPDE.

摘要

目的

研究叶绿酸（CHL）对反式苯并（a）芘 - 反式 - 7,8 - 二氢二醇 - 9,10 - 环氧化物（BPDE）诱导人支气管上皮细胞系（16HBE）恶性转化的抑制作用。

方法

在BPDE诱导细胞转化过程中，向培养基中加入10、50或100微摩尔/升的CHL，通过刀豆蛋白A凝集试验、非锚定依赖性生长测定和致瘤性测定来鉴定转化细胞的恶性程度。

结果

细胞培养25代后，CHL与BPDE联合处理组细胞凝集时间显著延长；CHL与BPDE联合处理组（每千分率分别为7.4、11.4和14.4）在软琼脂中的集落形成效率与单独用BPDE处理组（每千分率为19.6）相比，呈剂量依赖性显著降低（P<0.05）。CHL与BPDE联合处理组或单独用BPDE处理组的细胞在裸鼠体内均形成肿瘤，经组织病理学检查确诊为鳞状细胞癌。CHL与BPDE联合处理组（肿瘤体积分别为0.43±0.13立方厘米、0.22±0.04立方厘米和0.10±0.06立方厘米）的肿瘤体积与单独用BPDE处理组（1.71±0.37立方厘米）相比，显著减小（P<0.05）且呈剂量依赖性。