血清中苯丙氨酸的一种稳健的特异性酶介导检测方法的开发与验证。
Development and validation of a robust specific enzyme mediated assay for phenylalanine in serum.
作者信息
Campbell R S, Brearley G M, Varsani H, Morris H C, Milligan T P, Hall S K, Hammond P M, Price C P
机构信息
Department of Clinical Biochemistry, London Hospital Medical College, UK.
出版信息
Clin Chim Acta. 1992 Sep 30;210(3):197-210. doi: 10.1016/0009-8981(92)90205-5.
The specificity of a phenylalanine dehydrogenase, particularly with respect to cross reactivity toward tyrosine, has been shown to be pH dependent, being minimal at high pH. The dehydrogenase step has been coupled to colorimetric detection of NADH using a tetrazolium salt. The assay shows no significant cross reactivity towards a range of amino acids or drugs and correlates well with an established HPLC technique.
苯丙氨酸脱氢酶的特异性，尤其是对酪氨酸的交叉反应性，已被证明取决于pH值，在高pH值时最低。脱氢酶步骤已与使用四唑盐对NADH进行比色检测相耦合。该测定法对一系列氨基酸或药物没有明显的交叉反应性，并且与既定的HPLC技术相关性良好。
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