Rubinsztajn Renata, Wrońska Jolanta, Chazan Ryszarda
Katedra i Klinika Chorób Wewnetrznych Pneumonologii i Alergologii AM w Warszawie.
Pol Arch Med Wewn. 2003 Aug;110(2):849-54.
Leukotrienes (LTC4, LTD4, LTE4) belong to eicosanoids and they play important role in allergic inflammation. Leukotrienes are 5-lipooxygenaze products of arachinoid acid. It is known that concentration of LTE4 increases in patients with bronchial asthma, after some allergy provocation and in patients with bronchial asthma and intolerance of on steroids anti-inflammatory drugs. The aim of the study was estimated the urinary concentration of LTE4 in patients with bronchial asthma and intolerance of no steroids anti-inflammatory drugs.
The study group consisted of 21 patients with asthma and intolerance of non steroids antiinflammatory drugs (F 19, M 2) in age from 21 to 72 years old (mean = 49 +/- 14), with middle time of asthma duration mean = 13.4 +/- 12.9 years In study group 11 person had positive skin test, 7 nasal polyps, and 8 person positive family history of bronchial asthma. After oral provocation aspirin challenge in 5 subjects' aspirin induced asthma was confirmed, 3 persons were not qualified to test. Urinary concentration of LTE4 before and 24 h after aspirin provocation was analyzed in all the patients. Leukotriene were detected by enzymatic Leukotriene E4, EIA Kit, Cayman Chemical test.
In group of patients with aspirin asthma basic concentration of LTE4 was 416.6 +/- 374.4 pg/mL, and after provocation 496.6 +/- 485.3 pg/mL, in the group without sensitivity to aspirin appropriate 262.9 +/- 404.0 vs 261.2 +/- 259.66 pg/mL, and in the group disqualified to test 181.6 +/- 55.75 pg/mL.
1 Patients with aspirin asthma have higher concentration of LTE4. 2. Excretion of LTE4 in patients with aspirin induced asthma raised after oral aspirin provocation and higher level was detected is til 24 hours after challenge. 3. This results confirmed the role of cysteinic leukotrienes in pathogenesis of aspirin induced asthma.
白三烯（LTC4、LTD4、LTE4）属于类花生酸，在过敏性炎症中起重要作用。白三烯是花生四烯酸的5-脂氧合酶产物。已知支气管哮喘患者、经某些变应原激发后以及支气管哮喘且不耐受类固醇抗炎药的患者，其LTE4浓度会升高。本研究的目的是评估支气管哮喘且不耐受非类固醇抗炎药患者尿液中LTE4的浓度。
研究组由21例年龄在21至72岁（平均49±14岁）的哮喘且不耐受非类固醇抗炎药的患者组成（女性19例，男性2例），哮喘病程平均为13.4±12.9年。研究组中11人皮肤试验呈阳性，7人有鼻息肉，8人有支气管哮喘家族史阳性。5名受试者经口服激发试验（阿司匹林激发试验）后确诊为阿司匹林诱发哮喘，3人不符合试验条件。分析了所有患者在阿司匹林激发试验前及激发后24小时尿液中LTE4的浓度。白三烯通过酶促白三烯E4酶免疫分析试剂盒（开曼化学检测）进行检测。
阿司匹林哮喘组患者LTE4的基础浓度为416.6±374.4 pg/mL，激发后为496.6±485.3 pg/mL；对阿司匹林无敏感性的组相应为262.9±404.0 vs 261.2±259.66 pg/mL；不符合试验条件的组为181.6±55.75 pg/mL。
Zotero 插件